Team:UNIPV-Pavia/Methods Materials/LB

From 2009.igem.org

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*Cover and store at +4°C.
*Cover and store at +4°C.
*Always check for contaminations before using!
*Always check for contaminations before using!
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<br><br><br>'''Clean bottle or E-flask'''<br><br><br><br><br>
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'''Clean bottle or flask'''<hr color='white' width='70%'>
'''NaCl'''<hr color='white' width='70%'>
'''NaCl'''<hr color='white' width='70%'>
'''BactoTryptone'''<hr color='white' width='70%'>
'''BactoTryptone'''<hr color='white' width='70%'>

Revision as of 08:23, 29 June 2009

EthanolPVanimation.gif



Protocols



LB medium

(Estimated time: 10 min + 4 hours of autoclavation and cooling)

  • For a final volume of 1 l, put:
    • 10 g NaCl
    • 10 g BactoTryptone
    • 5 g Yeast extract
    • 15 g Agar (only for LB plates)
  • into 1 l of ddH2O.
  • Autoclave the solution.
  • Let it cool to ~40-45°C
  • Add the proper antibiotic if needed.
  • ONLY FOR PLATES:
    • Pour an homogenous layer of agar LB into Petri plates under the hood.
    • Let agar LB polymerase.
  • Cover and store at +4°C.
  • Always check for contaminations before using!










Clean bottle or flask
NaCl
BactoTryptone
Yeast extract
ddH2O
Agar




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