Team:UNIPV-Pavia/Methods Materials/LB

From 2009.igem.org

(Difference between revisions)
(→LB medium)
(→LB medium)
Line 53: Line 53:
*Cover and store at +4°C.
*Cover and store at +4°C.
*Always check for contaminations before using!
*Always check for contaminations before using!
-
<br><br><br><br><br><br><br><br>
 
<br></td><td class='beuta' align='center' valign='bottom' width='300px'>
<br></td><td class='beuta' align='center' valign='bottom' width='300px'>
<div align='center'>
<div align='center'>

Revision as of 08:23, 29 June 2009

EthanolPVanimation.gif



Protocols



LB medium

(Estimated time: 10 min + 4 hours of autoclavation and cooling)

  • For a final volume of 1 l, put:
    • 10 g NaCl
    • 10 g BactoTryptone
    • 5 g Yeast extract
    • 15 g Agar (only for LB plates)
  • into 1 l of ddH2O.
  • Autoclave the solution.
  • Let it cool to ~40-45°C
  • Add the proper antibiotic if needed.
  • ONLY FOR PLATES:
    • Pour an homogenous layer of agar LB into Petri plates under the hood.
    • Let agar LB polymerase.
  • Cover and store at +4°C.
  • Always check for contaminations before using!

Clean bottle or flask
NaCl
BactoTryptone
Yeast extract
ddH2O
Agar




Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/LB"