ULB/12 July 2009

From 2009.igem.org

(Difference between revisions)

Latest revision as of 21:55, 20 October 2009

iGEM Team:ULB-Brussels Wiki

July
M	T	W	T	F	S	S
		[http://2009.igem.org/ULB/1_July_2009 1]	[http://2009.igem.org/ULB/2_July_2009 2]	[http://2009.igem.org/wiki/index.php?title=ULB/3_July_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=ULB/4_July_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=ULB/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=ULB/6_July_2009&action=edit 6]	[http://2009.igem.org/ULB/7_July_2009 7]	[http://2009.igem.org/wiki/index.php?title=ULB/8_July_2009&action=edit 8]	[http://2009.igem.org/ULB/9_July_2009 9]	[http://2009.igem.org/wiki/index.php?title=ULB/10_July_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=ULB/11_July_2009&action=edit 11]	[http://2009.igem.org/ULB/12_July_2009 12]
[http://2009.igem.org/wiki/index.php?title=ULB/13_July_2009&action=edit 13]	[http://2009.igem.org/wiki/index.php?title=ULB/14_July_2009&action=edit 14]	[http://2009.igem.org/ULB/15_July_2009 15]	[http://2009.igem.org/wiki/index.php?title=ULB/16_July_2009&action=edit 16]	[http://2009.igem.org/wiki/index.php?title=ULB/17_July_2009&action=edit 17]	[http://2009.igem.org/wiki/index.php?title=ULB/18_July_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=ULB/19_July_2009&action=edit 19]
[http://2009.igem.org/ULB/20_July_2009 20]	[http://2009.igem.org/ULB/21_July_2009 21]	[http://2009.igem.org/ULB/22_July_2009 22]	[http://2009.igem.org/ULB/23_July_2009 23]	[http://2009.igem.org/ULB/24_July_2009 24]	[http://2009.igem.org/ULB/25_July_2009 25]	[http://2009.igem.org/wiki/index.php?title=ULB/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=ULB/27_July_2009&action=edit 27]	[http://2009.igem.org/wiki/index.php?title=ULB/28_July_2009&action=edit 28]	[http://2009.igem.org/ULB/29_July_2009 29]	[http://2009.igem.org/wiki/index.php?title=ULB/30_July_2009&action=edit 30]	[http://2009.igem.org/wiki/index.php?title=ULB/31_July_2009&action=edit 31]

August
M	T	W	T	F	S	S
					[http://2009.igem.org/wiki/index.php?title=ULB/1_August_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=ULB/2_August_2009&action=edit 2]
[http://2009.igem.org/ULB/3_August_2009 3]	[http://2009.igem.org/ULB/4_August_2009 4]	[http://2009.igem.org/wiki/index.php?title=ULB/5_August_2009&action=edit 5]	[http://2009.igem.org/ULB/6_August_2009 6]	[http://2009.igem.org/ULB/7_August_2009 7]	[http://2009.igem.org/wiki/index.php?title=ULB/8_August_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=ULB/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=ULB/10_August_2009&action=edit 10]	[http://2009.igem.org/ULB/11_August_2009 11]	[http://2009.igem.org/ULB/12_August_2009 12]	[http://2009.igem.org/ULB/13_August_2009 13]	[http://2009.igem.org/ULB/14_August_2009 14]	[http://2009.igem.org/wiki/index.php?title=ULB/15_August_2009&action=edit 15]	[http://2009.igem.org/wiki/index.php?title=ULB/16_August_2009&action=edit 16]
[http://2009.igem.org/ULB/17_August_2009 17]	[http://2009.igem.org/ULB/18_August_2009 18]	[http://2009.igem.org/ULB/19_August_2009 19]	[http://2009.igem.org/ULB/20_August_2009 20]	[http://2009.igem.org/ULB/21_August_2009 21]	[http://2009.igem.org/wiki/index.php?title=ULB/22_August_2009&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=ULB/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=ULB/24_August_2009&action=edit 24]	[http://2009.igem.org/ULB/25_August_2009 25]	[http://2009.igem.org/ULB/26_August_2009 26]	[http://2009.igem.org/wiki/index.php?title=ULB/27_August_2009&action=edit 27]	[http://2009.igem.org/ULB/28_August_2009 28]	[http://2009.igem.org/wiki/index.php?title=ULB/29_August_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=ULB/30_August_2009&action=edit 30]
[http://2009.igem.org/ULB/31_August_2009 31]

September
M	T	W	T	F	S	S
	[http://2009.igem.org/ULB/1_September_2009 1]	[http://2009.igem.org/wiki/index.php?title=ULB/2_September_2009&action=edit 2]	[http://2009.igem.org/ULB/3_September_2009 3]	[http://2009.igem.org/ULB/4_September_2009 4]	[http://2009.igem.org/ULB/5_September_2009 5]	[http://2009.igem.org/wiki/index.php?title=ULB/6_September_2009&action=edit 6]
[http://2009.igem.org/ULB/7_September_2009 7]	[http://2009.igem.org/wiki/index.php?title=ULB/8_September_2009&action=edit 8]	[http://2009.igem.org/ULB/9_September_2009 9]	[http://2009.igem.org/ULB/10_September_2009 10]	[http://2009.igem.org/ULB/11_September_2009 11]	[http://2009.igem.org/ULB/12_September_2009 12]	[http://2009.igem.org/wiki/index.php?title=ULB/13_September_2009&action=edit 13]
[http://2009.igem.org/ULB/14_September_2009 14]	[http://2009.igem.org/wiki/index.php?title=ULB/15_September_2009&action=edit 15]	[http://2009.igem.org/wiki/index.php?title=ULB/16_September_2009&action=edit 16]	[http://2009.igem.org/wiki/index.php?title=ULB/17_September_2009&action=edit 17]	[http://2009.igem.org/wiki/index.php?title=ULB/18_September_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=ULB/19_September_2009&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=ULB/20_September_2009&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=ULB/21_September_2009&action=edit 21]	[http://2009.igem.org/wiki/index.php?title=ULB/22_September_2009&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=ULB/23_September_2009&action=edit 23]	[http://2009.igem.org/wiki/index.php?title=ULB/24_September_2009&action=edit 24]	[http://2009.igem.org/wiki/index.php?title=ULB/25_September_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=ULB/26_September_2009&action=edit 26]	[http://2009.igem.org/wiki/index.php?title=ULB/27_September_2009&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=ULB/28_September_2009&action=edit 28]	[http://2009.igem.org/wiki/index.php?title=ULB/29_September_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=ULB/30_September_2009&action=edit 30]

July 12, 2009

Forward and reverse primers are designed and ordered.

Primers design for hfsG et hfsH genes

BBa_R0011 (promoter) :

PREFIX :

=> GAATTCATGAGATCT aattgtgagcggataaca

SUFFIX :

<= ctcgagttaggatcc TGTGCTCAGTATCTTGTT

RBS BBa_B0034 :

PREFIX :

=> GAATTCATGAGATCT AAAGAG

SUFFIX :

<= ctcgagttaggatcc TTTCTC

hfsG gene :

PREFIX :

=> GAATTCATGAGATCT atgaacgcgcccgtcaacgagctgcgactggagaacgccg

SUFFIX :

<= cagacggcctcgctgtaga

hfsH gene :

PREFIX :

=> atgccgatggagttcgagaag

SUFFIX :

<= ctcgagttaggatcc ttagagcccgatcc

RBS + RFP :

PREFIX :

=> GAATTCATGAGATCT aaagaggagaaatactag

SUFFIX :

<= ctcgagttaggatcc GCGATCTACACTAGCACT

BBa_B0015 terminator :

PREFIX :

=> GAATTCATGAGATCT ccaggcatcaaataaaac

SUFFIX :

<= ctcgagttaggatcc TATAAACGCAGAAAGGCC

Sequences

Promoter (55 bp)

aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca

RBS (12 pb)

aaagaggagaaa

RBS + RFP (681 bp)

aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgt
atggaaggttccgttaacggtcacgagttcgAaatcgaaggtgaaggtgaaggtcgtccgtacgaaggt
acccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg
cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcc
ttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggt
gctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa
accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatc
acctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggt
gcttaataacgctgatagtgctagtgtagatcgc

Terminator (129 bp)

ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

Plasmid (3165 bp)

ggatcctaactcgaggttacattgtcgatctgttcatggtgaacagctttaaatgcaccaaaaactcgt
aaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgat
atctaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagcc
ataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgag
ccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggt
gagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctga
tgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatc
catttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaa
tttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaa
ctcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgcctt
gtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaa
agacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttc
actaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcc
tttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacacc
ataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttc
tttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatg
ctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatct
caattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtcct
tttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctag
accctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatag
aataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtc
agttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccc
taaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatca
ggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggg
gtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccg
tcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagc
agttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggct
aatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattc
tcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcatt
actggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagttaccaatgcttaatcag
tgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat
aactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcacc
ggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagttt
gcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcag
ctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcctt
cggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgca
taattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt
ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca
tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttacc
gctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcac
cagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaa
atgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgag
cggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccatggtgcc
acctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaa
tttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcatgagatct

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 html body div.header div.banner {
 	height: 171px;
-	background-image: url(https://static.igem.org/mediawiki/2009/4/42/BannerUlb15.PNG);
+	background-image: url(https://static.igem.org/mediawiki/2009/0/0a/Logoulb1.png);
      background-repeat: no-repeat;
 }
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 					<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li>
 					<li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li>
-					<li><a class="mainlink" href="/Team:ULB-Brussels/Modeling">Modeling</a></li>
+					<li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li>
 					<li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li>
 					<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li>
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-table.calendar          { margin: 0; padding: 10px; }
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-table.month .heading td { padding:2px; background-color:#d4d4d4; color:#aaa; text-align:center; font-size:120%; font-weight:bold; }
-table.month .dow td     { color:#aaa; text-align:center; font-size:110%; }
-table.month td.today    { background-color:#ddd; }
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-    font-size: 8pt;
-    text-align: right;
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-.day-active { color:blue }
-.day-empty  { color:black }
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 |}

ULB/12 July 2009

From 2009.igem.org

Latest revision as of 21:55, 20 October 2009

Contents

July 12, 2009

Primers design for hfsG et hfsH genes

BBa_R0011 (promoter) :

RBS BBa_B0034 :

hfsG gene :

hfsH gene :

RBS + RFP :

BBa_B0015 terminator :

Sequences

Promoter (55 bp)

RBS (12 pb)

RBS + RFP (681 bp)

Terminator (129 bp)

Plasmid (3165 bp)