Team:UNIPV-Pavia/Methods Materials/XGal

(Difference between revisions)

Revision as of 11:42, 30 June 2009

Protocols

(Estimated time: 20 min)

The principle is that X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) turns blue when reacts with beta-galactosidase.
Mix 20 ul X-Gal 20 mg/ml and 80 ul SOC and spread on LB+amp plates (X-Gal and DMF are toxic, use face-mask for your safety!!! X-Gal is light-sensitive, remember to keep it in the dark, when possible)
Incubate with 200 ul bacteria
Result: blue colonies express LacZ, while white colonies don't.

@@ Line 37: / Line 37: @@
 <br>
 <br>
-'''Materials needed:'''
+<table width='80%'><tr><td>
+*The principle is that X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) turns blue when reacts with beta-galactosidase.
+*Mix 20 ul X-Gal 20 mg/ml and 80 ul SOC and spread on LB+amp plates (X-Gal and DMF are toxic, use face-mask for your safety!!! X-Gal is light-sensitive, remember to keep it in the dark, when possible)
+*Incubate with 200 ul bacteria
+*Result: blue colonies express LacZ, while white colonies don't.
+<br><br><br><br><br><br><br><br>
+<br></td><td class='beuta' align='center' valign='bottom' width='300px'>
+<div align='center'>
+<table border='1'>
+<tr><td>
 *'''40 mg/ml X-gal in DMF'''
 *'''SOC medium'''
@@ Line 43: / Line 52: @@
 *'''Face mask'''
 <br>
+</td></tr></table></div><br><br><br><br>
-*The principle is that X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) turns blue when reacts with beta-galactosidase.
+</td></tr></table>
-*Mix 20 ul X-Gal 20 mg/ml and 80 ul SOC and spread on LB+amp plates (X-Gal and DMF are toxic, use face-mask for your safety!!! X-Gal is light-sensitive, remember to keep it in the dark, when possible)
-*Incubate with 200 ul bacteria
-*Result: blue colonies express LacZ, while white colonies don't.