Team:UNIPV-Pavia/Protocols/Precipitation

From 2009.igem.org

(Difference between revisions)
(→DNA precipitation with sodium acetate)
(→DNA precipitation with sodium acetate)
Line 36: Line 36:
<br>
<br>
<br>
<br>
-
'''Materials needed:'''
+
<table width='80%'><tr><td>
-
 
+
-
*'''Sodium acetate'''
+
-
*'''Absolute ethanol'''
+
-
*'''Ethanol 70%'''
+
-
*'''ddH2O'''
+
-
*'''Previously cut plasmid'''
+
-
<br>
+
*We use it only if the miniprep did not give us a high yield of DNA  
*We use it only if the miniprep did not give us a high yield of DNA  
*Add 1/10 digestion volume of sodium acetate 3 M
*Add 1/10 digestion volume of sodium acetate 3 M
Line 55: Line 48:
*Air dry pellet until ethanol is totally removed
*Air dry pellet until ethanol is totally removed
*Elute with 5 µl ddH2O
*Elute with 5 µl ddH2O
-
<br>
+
<br><br><br><br><br><br><br><br>
 +
<br></td><td class='beuta' align='center' valign='bottom' width='300px'>
 +
<div align='center'>
 +
<table border='1'>
 +
<tr><td>
 +
*'''Sodium acetate'''
 +
*'''Absolute ethanol'''
 +
*'''Ethanol 70%'''
 +
*'''ddH2O'''
 +
*'''Previously cut plasmid'''
 +
</td></tr></table></div><br><br><br><br>
 +
</td></tr></table

Revision as of 11:55, 30 June 2009

EthanolPVanimation.gif



Protocols

DNA precipitation with sodium acetate

(estimated time: 1 hour)

</table
  • We use it only if the miniprep did not give us a high yield of DNA
  • Add 1/10 digestion volume of sodium acetate 3 M
  • Add 2.5 digestion volume of absolute ethanol
  • Freeze at -80°C for 30 min
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Decant supernatant
  • Add 50 µl of 70% ethanol
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Remove all supernatant with a pipette
  • Air dry pellet until ethanol is totally removed
  • Elute with 5 µl ddH2O










  • Sodium acetate
  • Absolute ethanol
  • Ethanol 70%
  • ddH2O
  • Previously cut plasmid




Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Protocols/Precipitation"