Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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The insert looks shifted upwards but a little amount so we didn't achieve to ligate the iptg to cox+ogr+ter | The insert looks shifted upwards but a little amount so we didn't achieve to ligate the iptg to cox+ogr+ter | ||
- | this is strange because the plasmid maintained its size so the promoter is not there. | + | this is strange because the plasmid maintained its size so the promoter is not there.I've checked the plasmid size an it is 2079 bp so the plasmid doesn't have the promoter. |
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- | I've checked the plasmid size an it is 2079 bp so the plasmid doesn't have the promoter. | + | |
Going back further when I digested for the first time plasmid 12 I did it with SpeI/PstI to allow | Going back further when I digested for the first time plasmid 12 I did it with SpeI/PstI to allow | ||
- | promoter to be at the beginning of the construction. | + | promoter to be at the beginning of the construction.The insert cox+ogr+ter was digested XbaI/PstI so I think the unique alternative is that promoter ipteg wasn't |
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- | The insert cox+ogr+ter was digested XbaI/PstI so I think the unique alternative is that promoter ipteg wasn't | + | |
in the plasmid. | in the plasmid. | ||
Revision as of 04:25, 21 October 2009