Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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To test for the activity of the multipromoter that we have designed and sent to synthesis we are going to | To test for the activity of the multipromoter that we have designed and sent to synthesis we are going to | ||
- | transform E. coli | + | transform E. coli [https://2009.igem.org/Team:LCG-UNAM-Mexico/Resources/Strains#Expression_Strains BL21(DE3)plysS] |
+ | This strain uses a T7 phage polymerase that is controlled via IPTG. The multipromoter is composed of T7 & T3 promoters. | ||
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+ | Transformed cells were platted on LB medium with Kn and IPTG .5 mM | ||
+ | == October 6, 2009 == | ||
+ | Using an aproppiate microscope we tried to see florescence and we saw a little of florescence but we wer not sure if it was an endogenous florescence or GFP. We must put a control to be sure that what we are looking is GFP, so we need to use | ||
+ | a BL21 that dosen't have the plasmid that contains our construction | ||
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Revision as of 04:50, 21 October 2009