"
Team:IIT Bombay India/Notebook
From 2009.igem.org
| Line 25: | Line 25: | ||
[[Week13]] (Aug, 3-8) Transformations in ''E.coli'' MG1655 (lac I deleted) | [[Week13]] (Aug, 3-8) Transformations in ''E.coli'' MG1655 (lac I deleted) | ||
| - | [[Week14]] (Aug, 10-15) | + | [[Week14]] (Aug, 10-15) Confirmation of the transformants |
| - | [[Week15]] (Aug, 17-22) | + | [[Week15]] (Aug, 17-22) Preparation for time course expression analysis |
| - | [[Week16]] (Aug, 24-29) FACS | + | [[Week16]] (Aug, 24-29) FACS time course |
| - | [[Week17]] (Aug-Sep, 31-5) | + | [[Week17]] (Aug-Sep, 31-5) FACS: different IPTG conc. |
| - | [[Week18]] (Sep, 7-13) | + | [[Week18]] (Sep, 7-13) Growth curves for all the four strains |
[[Week19]] (Sep, 14-20) Growth curve on Lactose (ptet A and plac K) | [[Week19]] (Sep, 14-20) Growth curve on Lactose (ptet A and plac K) | ||
Revision as of 09:05, 21 October 2009
Week1 FACS Trial
Week2 (May, 18-23) Preparation
Week3 (May, 25-30) FACS
Week4 (Jun, 1-6) Growth Curve
Week5 (Jun, 8-13) FACS:YFP signal
Week6 (Jun, 15-20) FACS:CFP signal
Week7 (Jun, 22-27) FACS repeat
Week8 (Jun-Jul, 29-4) Req.:Transformation
Week9 (Jul, 6-11) Cultured Host
Week10 (Jul, 13-18) Transformation of 4 strains
Week11 (Jul, 20-24) FACS:Constructs
Week12 (Jul, 27-31) FACS
Week13 (Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)
Week14 (Aug, 10-15) Confirmation of the transformants
Week15 (Aug, 17-22) Preparation for time course expression analysis
Week16 (Aug, 24-29) FACS time course
Week17 (Aug-Sep, 31-5) FACS: different IPTG conc.
Week18 (Sep, 7-13) Growth curves for all the four strains
Week19 (Sep, 14-20) Growth curve on Lactose (ptet A and plac K)
Week20 (Sep, 21-27) Growth curve on Lactose (plac A and ptet K)
Week21 (Sep-Oct, 28-3) Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)
Week22 (Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)
Week23 (Oct, 12-17) Growth curve on Lactose (ptet A and plac K)
Week24 (Oct, 18-21) Data compilation and analysis, wiki uploads
