Team:UNIPV-Pavia/Methods Materials/LB

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(New page: {{UNIPV-Pavia/Protocols}} <br> == '''LB medium''' == ''(Estimated time: 10 min + 4 hours of autoclavation and cooling)'' <br> <br> '''Materials needed:''' *'''NaCl''' *'''BactoTryptone'''...)
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== '''LB medium''' ==  
== '''LB medium''' ==  
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'''Materials needed:'''
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*'''NaCl'''
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*'''BactoTryptone'''
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*'''Yeast extract '''
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*'''ddH2O'''
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*'''Agar'''
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*'''Clean bottle or E-flask'''
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*For a final volume of 1 l, put:
*For a final volume of 1 l, put:
**10 g NaCl
**10 g NaCl
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*Cover and store at +4°C.
*Cover and store at +4°C.
*Always check for contaminations before using!
*Always check for contaminations before using!
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<tr bgcolor='yellow'><td>INGREDIENTS:
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'''Clean bottle or flask'''<hr color='white' width='70%'>
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'''NaCl'''<hr color='white' width='70%'>
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'''BactoTryptone'''<hr color='white' width='70%'>
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'''Yeast extract '''<hr color='white' width='70%'>
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'''ddH2O'''<hr color='white' width='70%'>
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'''Agar''' </font>
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Latest revision as of 10:25, 21 October 2009

EthanolPVanimation.gif



Protocols



LB medium

(Estimated time: 10 min + 4 hours of autoclavation and cooling)

  • For a final volume of 1 l, put:
    • 10 g NaCl
    • 10 g BactoTryptone
    • 5 g Yeast extract
    • 15 g Agar (only for LB plates)
  • into 1 l of ddH2O.
  • Autoclave the solution.
  • Let it cool to ~40-45°C
  • Add the proper antibiotic if needed.
  • ONLY FOR PLATES:
    • Pour an homogenous layer of agar LB into Petri plates under the hood.
    • Let agar LB polymerase.
  • Cover and store at +4°C.
  • Always check for contaminations before using!










INGREDIENTS:

Clean bottle or flask
NaCl
BactoTryptone
Yeast extract
ddH2O

Agar





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