Team:UNIPV-Pavia/Methods Materials/LB

From 2009.igem.org

(Difference between revisions)
(LB medium)
 
(40 intermediate revisions not shown)
Line 1: Line 1:
{{UNIPV-Pavia/StyleCss}}
{{UNIPV-Pavia/StyleCss}}
<html>
<html>
-
<head>
+
 
-
<link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
+
-
</head>
+
-
<body>
+
<table width="100%">
<table width="100%">
   <tr>
   <tr>
<td align="center">
<td align="center">
-
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/c/c2/UNIPV_title_MandM.jpg" ></img>
+
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img>
</td>
</td>
   </tr>
   </tr>
</table>
</table>
-
</body>
+
 
</html>
</html>
Line 25: Line 22:
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     </td>
     </td>
 +
    <td width="50px"></td>
</html>
</html>
   </tr>
   </tr>
Line 38: Line 36:
<br>
<br>
<br>
<br>
-
<table><tr><td>
+
<table width='80%'><tr><td>
*For a final volume of 1 l, put:
*For a final volume of 1 l, put:
**10 g NaCl
**10 g NaCl
Line 53: Line 51:
*Cover and store at +4°C.
*Cover and store at +4°C.
*Always check for contaminations before using!
*Always check for contaminations before using!
-
<br></td><td align='right'>
+
<br><br><br><br><br><br><br><br>
-
<table bgcolor='red' size='30%' valign='top'>
+
<br></td><td align='center' valign='top'  width='300px'>
-
<tr><td bgcolor='red' valign='top'>'''Materials needed:'''
+
 
-
*'''NaCl'''
+
<table border='1'>
-
*'''BactoTryptone'''
+
<tr bgcolor='yellow'><td>INGREDIENTS:
-
*'''Yeast extract '''
+
</td></tr>
-
*'''ddH2O'''
+
<tr><td>
-
*'''Agar'''
+
<font class='label'>
-
*'''Clean bottle or E-flask'''
+
'''Clean bottle or flask'''<hr color='white' width='70%'>
-
<br>
+
'''NaCl'''<hr color='white' width='70%'>
-
</td></tr></table>
+
'''BactoTryptone'''<hr color='white' width='70%'>
 +
'''Yeast extract '''<hr color='white' width='70%'>
 +
'''ddH2O'''<hr color='white' width='70%'>
 +
'''Agar''' </font>
 +
</td></tr></table><br><br><br><br>
</td></tr></table>
</td></tr></table>

Latest revision as of 10:25, 21 October 2009

EthanolPVanimation.gif



Protocols



LB medium

(Estimated time: 10 min + 4 hours of autoclavation and cooling)

  • For a final volume of 1 l, put:
    • 10 g NaCl
    • 10 g BactoTryptone
    • 5 g Yeast extract
    • 15 g Agar (only for LB plates)
  • into 1 l of ddH2O.
  • Autoclave the solution.
  • Let it cool to ~40-45°C
  • Add the proper antibiotic if needed.
  • ONLY FOR PLATES:
    • Pour an homogenous layer of agar LB into Petri plates under the hood.
    • Let agar LB polymerase.
  • Cover and store at +4°C.
  • Always check for contaminations before using!










INGREDIENTS:

Clean bottle or flask
NaCl
BactoTryptone
Yeast extract
ddH2O

Agar