Team:UNIPV-Pavia/Methods Materials/Glycerol stock

From 2009.igem.org

(Difference between revisions)
(→Glycerol stocks)
(→Glycerol stocks)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
-
{{UNIPV-Pavia/StyleCss}}
 
<html>
<html>
-
<head>
+
 
-
<link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
+
-
</head>
+
-
<body>
+
<table width="100%">
<table width="100%">
   <tr>
   <tr>
<td align="center">
<td align="center">
-
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/c/c2/UNIPV_title_MandM.jpg" ></img>
+
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img>
</td>
</td>
   </tr>
   </tr>
</table>
</table>
-
</body>
+
 
</html>
</html>
Line 25: Line 21:
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     </td>
     </td>
 +
    <td width="50px"></td>
</html>
</html>
   </tr>
   </tr>
Line 41: Line 38:
*Leave at -20°C for one day
*Leave at -20°C for one day
*Move to -80°C the day after
*Move to -80°C the day after
-
<br><br><br><br><br><br><br><br>
+
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
-
<br></td><td class='glicerolo' align='center' valign='bottom' width='300px'>
+
<br></td><td align='center' valign='top' width='300px'>
-
<table class='in_glicerolo' border='1'>
+
<table border='1'>
 +
<tr bgcolor='yellow'><td>INGREDIENTS:
 +
</td></tr>
<tr><td>
<tr><td>
<font class='label'>
<font class='label'>

Latest revision as of 10:27, 21 October 2009

EthanolPVanimation.gif



Protocols


Glycerol stocks

(estimated time: 15 min + freezing time)

  • Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after

















INGREDIENTS:

o/n or in exp phase bacterial culture

80% glycerol





Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Glycerol_stock"