Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction

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(Plasmid extraction)
 
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     <font face="Pristina" size="50" ><b>Protocols</b></font>
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*Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]
*Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]
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<tr bgcolor='yellow'><td>INGREDIENTS:
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'''5 ml or 9 ml (for higher yields) overnight mini-culture <hr color='white' width='70%'>
'''5 ml or 9 ml (for higher yields) overnight mini-culture <hr color='white' width='70%'>
'''QIAGEN QIAprep Spin Miniprep Kit'''</font>
'''QIAGEN QIAprep Spin Miniprep Kit'''</font>
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Latest revision as of 10:28, 21 October 2009

EthanolPVanimation.gif



Protocols


Plasmid extraction

(Estimated time: 1 hour)

  • Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]










INGREDIENTS:

5 ml or 9 ml (for higher yields) overnight mini-culture

QIAGEN QIAprep Spin Miniprep Kit