Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction

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(New page: <h1>Plasmid extraction</h1> ''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)'' <br> <br> '''Materials needed:''' *'''5 ml ...)
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''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)''
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    <font face="Pristina" size="50" ><b>Protocols</b></font>
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=='''Plasmid extraction'''==
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''(Estimated time: 1 hour)''
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'''Materials needed:'''
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*'''5 ml or 9 ml (for higher yields) overnight mini-culture
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*Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]
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*'''QIAGEN Plasmid Mini Kit or QIAGEN QIAprep Spin Miniprep Kit'''
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*Follow QIAGEN standard protocol: [http://www1.qiagen.com/literature/render.aspx?id=369&tp=4 QIAGEN Plasmid Mini Kit protocol] or [http://www1.qiagen.com/literature/render.aspx?id=370&tp=4 QIAGEN QIAprep Spin Miniprep protocol]
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*We used QIAGEN Plasmid Mini Kit in the first 2 weeks of our activity to perform miniprep, when we didn't have QIAGEN QIAprep Spin Miniprep Kit at our disposal. Then, we always used QIAGEN QIAprep Spin Miniprep Kit for its higher yield.
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<tr bgcolor='yellow'><td>INGREDIENTS:
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'''5 ml or 9 ml (for higher yields) overnight mini-culture <hr color='white' width='70%'>
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'''QIAGEN QIAprep Spin Miniprep Kit'''</font>
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Latest revision as of 10:28, 21 October 2009

EthanolPVanimation.gif



Protocols


Plasmid extraction

(Estimated time: 1 hour)

  • Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]










INGREDIENTS:

5 ml or 9 ml (for higher yields) overnight mini-culture

QIAGEN QIAprep Spin Miniprep Kit





Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/PlasmidExtraction"