Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction

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(→Plasmid extraction)
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     <font face="Pristina" size="50" ><b>Protocols</b></font>
     <font face="Pristina" size="50" ><b>Protocols</b></font>
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=='''Plasmid extraction'''==
=='''Plasmid extraction'''==
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''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)''
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''(Estimated time: 1 hour)''
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*Follow QIAGEN standard protocol: [www.biochem.arizona.edu/AZ.../QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]
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*Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]
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*'''5 ml or 9 ml (for higher yields) overnight mini-culture
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*'''QIAGEN QIAprep Spin Miniprep Kit'''
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'''5 ml or 9 ml (for higher yields) overnight mini-culture <hr color='white' width='70%'>
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'''QIAGEN QIAprep Spin Miniprep Kit'''</font>
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Latest revision as of 10:28, 21 October 2009

EthanolPVanimation.gif



Protocols


Plasmid extraction

(Estimated time: 1 hour)

  • Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]










INGREDIENTS:

5 ml or 9 ml (for higher yields) overnight mini-culture

QIAGEN QIAprep Spin Miniprep Kit





Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/PlasmidExtraction"