Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction

From 2009.igem.org

(Difference between revisions)
(→Plasmid extraction)
(→Plasmid extraction)
 
(5 intermediate revisions not shown)
Line 1: Line 1:
-
{{UNIPV-Pavia/StyleCss}}
 
<html>
<html>
-
<head>
+
 
-
<link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
+
-
</head>
+
-
<body>
+
<table width="100%">
<table width="100%">
   <tr>
   <tr>
<td align="center">
<td align="center">
-
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/c/c2/UNIPV_title_MandM.jpg" ></img>
+
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img>
</td>
</td>
   </tr>
   </tr>
</table>
</table>
-
</body>
+
 
</html>
</html>
Line 25: Line 21:
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     </td>
     </td>
 +
    <td width="50px"></td>
</html>
</html>
   </tr>
   </tr>
Line 41: Line 38:
*Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]
*Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]
<br><br><br><br><br><br><br><br>
<br><br><br><br><br><br><br><br>
-
<br></td><td align='center' valign='bottom' width='300px'>
+
<br></td><td align='center' valign='top' width='300px'>
-
<div align='center'>
+
 
<table border='1'>
<table border='1'>
 +
<tr bgcolor='yellow'><td>INGREDIENTS:
 +
</td></tr>
<tr><td>
<tr><td>
-
*'''5 ml or 9 ml (for higher yields) overnight mini-culture
+
<font class='label'>
-
*'''QIAGEN QIAprep Spin Miniprep Kit'''
+
'''5 ml or 9 ml (for higher yields) overnight mini-culture <hr color='white' width='70%'>
-
</td></tr></table></div><br><br><br><br>
+
'''QIAGEN QIAprep Spin Miniprep Kit'''</font>
-
</td></tr></table>
+
</td></tr></table><br><br><br><br>

Latest revision as of 10:28, 21 October 2009

EthanolPVanimation.gif



Protocols


Plasmid extraction

(Estimated time: 1 hour)

  • Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]










INGREDIENTS:

5 ml or 9 ml (for higher yields) overnight mini-culture

QIAGEN QIAprep Spin Miniprep Kit





Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/PlasmidExtraction"