Team:UNIPV-Pavia/Methods Materials/Electrophoresis
From 2009.igem.org
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+ | {{UNIPV-Pavia/StyleCss}} | ||
<html> | <html> | ||
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<tr> | <tr> | ||
<td align="center"> | <td align="center"> | ||
- | <img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/ | + | <img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img> |
</td> | </td> | ||
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<font face="Pristina" size="50" ><b>Protocols</b></font> | <font face="Pristina" size="50" ><b>Protocols</b></font> | ||
</td> | </td> | ||
+ | <td width="50px"></td> | ||
</html> | </html> | ||
</tr> | </tr> | ||
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*Take a picture of the gel, if needed (not when bands have to be cut!!!) | *Take a picture of the gel, if needed (not when bands have to be cut!!!) | ||
<br><br><br><br><br><br><br><br> | <br><br><br><br><br><br><br><br> | ||
- | <br></td><td align='center' valign=' | + | <br></td><td align='center' valign='top' width='300px'> |
- | + | ||
<table border='1'> | <table border='1'> | ||
+ | <tr bgcolor='yellow'><td>INGREDIENTS: | ||
+ | </td></tr> | ||
<tr><td> | <tr><td> | ||
<font class='label'> | <font class='label'> | ||
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'''Ethidium bromide'''<hr color='white' width='70%'> | '''Ethidium bromide'''<hr color='white' width='70%'> | ||
'''Loading buffer 10x Blue Juice, Invitrogen'''<hr color='white' width='70%'> | '''Loading buffer 10x Blue Juice, Invitrogen'''<hr color='white' width='70%'> | ||
- | '''TBE (Tris/Borate/EDTA buffer) 5x (final volume 1 liter)''' | + | '''TBE (Tris/Borate/EDTA buffer) 5x (final volume 1 liter)''' |
- | + | *'''54 gr Tris''' | |
- | + | *'''27.5 gr Borate'''<hr color='white' width='70%'> | |
'''20 ml EDTA 0.5 M (pH 8)'''<hr color='white' width='70%'> | '''20 ml EDTA 0.5 M (pH 8)'''<hr color='white' width='70%'> | ||
'''Marker (1 kb DNA Ladder, Promega)'''<hr color='white' width='70%'> | '''Marker (1 kb DNA Ladder, Promega)'''<hr color='white' width='70%'> | ||
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'''Electrophoresis apparatus'''<hr color='white' width='70%'> | '''Electrophoresis apparatus'''<hr color='white' width='70%'> | ||
'''Transilluminator'''</font> | '''Transilluminator'''</font> | ||
- | </td></tr></table | + | </td></tr></table><br><br><br><br> |
</td></tr></table> | </td></tr></table> | ||
Latest revision as of 10:30, 21 October 2009
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Electrophoresis
(estimated time: 2 hours)
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- NOTE: when not specified, the marker has the following pattern:
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