Team:UNIPV-Pavia/Protocols/Precipitation
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(New page: <h1>DNA precipitation with sodium acetate</h1> ''(estimated time: 1 hour)'' <br> <br> '''Materials needed:''' *'''sodium acetate''' *'''absolute ethanol''' *'''ethanol 70%''' *'''ddH2O'''...) |
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+ | <font face="Pristina" size="50" ><b>Protocols</b></font> | ||
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+ | <td> {{UNIPV-Pavia/Protocols}}</td> | ||
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+ | == '''DNA precipitation with sodium acetate''' == | ||
''(estimated time: 1 hour)'' | ''(estimated time: 1 hour)'' | ||
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- | + | *Add 1/10 DNA solution volume of sodium acetate 3 M | |
- | + | *Add 2.5 DNA solution volume of absolute ethanol | |
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- | *Add 1/10 | + | |
- | *Add 2.5 | + | |
*Freeze at -80°C for 30 min | *Freeze at -80°C for 30 min | ||
*Centrifuge at 13000 rpm, 4°C for 20 min | *Centrifuge at 13000 rpm, 4°C for 20 min | ||
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*Remove all supernatant with a pipette | *Remove all supernatant with a pipette | ||
*Air dry pellet until ethanol is totally removed | *Air dry pellet until ethanol is totally removed | ||
- | *Elute with 5 µl ddH2O | + | *Elute with 5-10 µl of ddH2O |
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+ | <br></td><td align='center' valign='top' width='300px'> | ||
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+ | <tr bgcolor='yellow'><td>INGREDIENTS: | ||
+ | </td></tr> | ||
+ | <tr><td> | ||
+ | <font class='label'> | ||
+ | '''Sodium acetate'''<hr color='white' width='70%'> | ||
+ | '''Absolute ethanol'''<hr color='white' width='70%'> | ||
+ | '''Ethanol 70%'''<hr color='white' width='70%'> | ||
+ | '''ddH2O'''<hr color='white' width='70%'> | ||
+ | '''DNA sample'''</font> | ||
+ | </td></tr></table><br><br><br><br> | ||
+ | </td></tr></table> |
Latest revision as of 10:32, 21 October 2009
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DNA precipitation with sodium acetate
(estimated time: 1 hour)
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