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Team:UNIPV-Pavia/Methods Materials/Ligation
From 2009.igem.org
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<font face="Pristina" size="50" ><b>Protocols</b></font> | <font face="Pristina" size="50" ><b>Protocols</b></font> | ||
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**Heat ligation at 65°C for 10 min. | **Heat ligation at 65°C for 10 min. | ||
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| + | <tr bgcolor='yellow'><td>INGREDIENTS: | ||
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| - | + | '''Roche T4 Ligase'''<hr color='white' width='70%'> | |
| - | + | '''10X Roche T4 Ligase Buffer'''<hr color='white' width='70%'> | |
| - | </td></tr></table | + | '''ddH2O'''</font> |
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Latest revision as of 10:33, 21 October 2009
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Ligation
(estimated time: 20 min + 12-16 hours overnight incubation)
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