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Team:Wash U/Calendar-Home/1 July 2009
From 2009.igem.org
(Difference between revisions)
| Line 10: | Line 10: | ||
Miniprep #2: | Miniprep #2: | ||
| + | <table> | ||
| + | <tr> | ||
| + | <td> | ||
1) BBa_I15010 pSB2K3 | 1) BBa_I15010 pSB2K3 | ||
| Line 33: | Line 36: | ||
12) plasmid pRKCBC3 | 12) plasmid pRKCBC3 | ||
| + | |||
| + | <td> | ||
| + | |||
| + | 158 ng/ul | ||
| + | |||
| + | 210 ng/ul | ||
| + | |||
| + | 62.5 ng/ul | ||
| + | |||
| + | 325 ng/ul | ||
| + | |||
| + | 280 ng/ul | ||
| + | |||
| + | 145 ng/ul | ||
| + | |||
| + | 665 ng/ul | ||
| + | |||
| + | 308 ng/ul | ||
| + | |||
| + | 275 ng/ul | ||
| + | |||
| + | 338 ng/ul | ||
| + | |||
| + | 168 ng/ul | ||
| + | |||
| + | 272 ng/ul | ||
| + | |||
| + | </table> | ||
| + | |||
| + | <pre> | ||
| + | *Note: Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma) | ||
| + | </pre> | ||
Revision as of 16:04, 2 July 2009
-Miniprep #2 to obtain more biobrick DNA
-New gel with digested vs. undigested plasmids
-Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run these on gel tomorrow
Miniprep #2:
|
1) BBa_I15010 pSB2K3 2) BBa_E0240 pSB1A2 3) BBa_R0082 pSB1A2 4) plasmid pSB1T3 5) plasmid pSB1K3 6) BBa_B0034 pSB1A2 7) plasmid pSB1AT3 8) plasmid pSB1A3 9) plasmid pucHS27 10) plasmid pSB1AC3 11) BBa_B0015 pSB1AK3 12) plasmid pRKCBC3 |
158 ng/ul 210 ng/ul 62.5 ng/ul 325 ng/ul 280 ng/ul 145 ng/ul 665 ng/ul 308 ng/ul 275 ng/ul 338 ng/ul 168 ng/ul 272 ng/ul |
*Note: Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma)
