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Team:Kyoto/CiC/Results
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===Result of Subgoal C=== | ===Result of Subgoal C=== | ||
====sig-GFP expression in HeLa==== | ====sig-GFP expression in HeLa==== | ||
| - | + | To confirm the function of the signal sequence for importing protein into mitochondria, | |
| - | compared the expressioin pattern of sig-GFP or GFP with mitotracker signal. | + | we compared the expressioin pattern of sig-GFP or GFP with mitotracker signal. |
The GFP signal was detected throughout the cell except for the black granules in the cytoplasm or the nuclear, | The GFP signal was detected throughout the cell except for the black granules in the cytoplasm or the nuclear, | ||
while sig-GFP signal showed the string-like pattern in the cytoplasm. The black granules in the cytoplasm observed | while sig-GFP signal showed the string-like pattern in the cytoplasm. The black granules in the cytoplasm observed | ||
in the GFP-expressing cell were stained by mitotracker, and the result indicated that the GFP was | in the GFP-expressing cell were stained by mitotracker, and the result indicated that the GFP was | ||
normally eliminated from mitochondria (Fig. NUMBER, GFP and mitotracker merged). In case of sig-GFP, | normally eliminated from mitochondria (Fig. NUMBER, GFP and mitotracker merged). In case of sig-GFP, | ||
| - | mitochondria stained by a mitotracker and the GFP signal showed almost the same pattern | + | mitochondria stained by a mitotracker and the GFP signal showed almost the same pattern. |
the yellow color in the merge images (Fig. Number, sig-GFP and mitotracker merged) suggested that | the yellow color in the merge images (Fig. Number, sig-GFP and mitotracker merged) suggested that | ||
| - | the sig-GFP and mitochondria were | + | the sig-GFP and mitochondria were colocalized in the HeLa cell. |
We, consequently, our constructed signal sequence has the function of importing protein into mitochondria as expected. | We, consequently, our constructed signal sequence has the function of importing protein into mitochondria as expected. | ||
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Revision as of 14:39, 21 October 2009
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Results & Discussion
Result of Subgoal A
Result of Subgoal B
Result of Subgoal C
sig-GFP expression in HeLa
To confirm the function of the signal sequence for importing protein into mitochondria, we compared the expressioin pattern of sig-GFP or GFP with mitotracker signal. The GFP signal was detected throughout the cell except for the black granules in the cytoplasm or the nuclear, while sig-GFP signal showed the string-like pattern in the cytoplasm. The black granules in the cytoplasm observed in the GFP-expressing cell were stained by mitotracker, and the result indicated that the GFP was normally eliminated from mitochondria (Fig. NUMBER, GFP and mitotracker merged). In case of sig-GFP, mitochondria stained by a mitotracker and the GFP signal showed almost the same pattern. the yellow color in the merge images (Fig. Number, sig-GFP and mitotracker merged) suggested that the sig-GFP and mitochondria were colocalized in the HeLa cell. We, consequently, our constructed signal sequence has the function of importing protein into mitochondria as expected.
