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Uppsala-Sweden/6 October 2009
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(New page: {{Uppsala-Sweden Template}} ==Tri-Parental Mating== To prepare for the moving of constructs to synecocytis we prepared digestions of the shuttle vector. ....................... {{Uppsa...) |
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To prepare for the moving of constructs to synecocytis we prepared digestions of the shuttle vector. | To prepare for the moving of constructs to synecocytis we prepared digestions of the shuttle vector. | ||
| - | . | + | Digestion of the antisense RNA constructs with (P+T) for ligation into the shuttle vector, pSB1AXK. |
| + | |||
| + | ==Plasmid prep== | ||
| + | |||
| + | Plasmid preps were performed for the constructs, | ||
| + | |||
| + | RPRYT 1 | ||
| + | RPRAT 3 | ||
| + | RPRYT 3 | ||
| + | PRKRYT 5 | ||
| + | PRPRAT 1 | ||
| + | PRPRAT 3 | ||
| + | PRKRYT 2 | ||
| + | RPRAT 5 | ||
| + | |||
| + | The concentration varied between 400-80 µg/ml | ||
{{Uppsala-Sweden_Footer}} | {{Uppsala-Sweden_Footer}} | ||
Latest revision as of 15:27, 21 October 2009
Tri-Parental Mating
To prepare for the moving of constructs to synecocytis we prepared digestions of the shuttle vector.
Digestion of the antisense RNA constructs with (P+T) for ligation into the shuttle vector, pSB1AXK.
Plasmid prep
Plasmid preps were performed for the constructs,
RPRYT 1 RPRAT 3 RPRYT 3 PRKRYT 5 PRPRAT 1 PRPRAT 3 PRKRYT 2 RPRAT 5
The concentration varied between 400-80 µg/ml
