Duke University/16 June 2009

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(New page: Did PCR with Bioplastics #2 as template and Phusion mix Ran gels to check PCRs)
 
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Did PCR with Bioplastics #2 as template and Phusion mix
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[[Team:Duke | Return]]
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Ran gels to check PCRs
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==June 16, 2009==
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*E: PCR with Bioplastics #2 as template and Phusion mix (done by Maggie), Gels to check PCRs
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<pre>
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2x Phusion mix 25 ul
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forward primer 2.5 ul
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reverse primer 2.5 ul
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DNA template (plasmid) 1 ul
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H2O 19 ul
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50 ul
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</pre>
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*R: No bands for fragments 3, Bands for fragment 4
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*C: Cut out bands of fragment 4, PCR again to obtain fragment 3
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*E: Measured concentration of DNA fragments 1 and 2
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*R: Concentrations okay
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*C: Do assembly PCR once enough of fragments 3 and 4 obtained

Latest revision as of 16:40, 21 October 2009

Return

June 16, 2009

  • E: PCR with Bioplastics #2 as template and Phusion mix (done by Maggie), Gels to check PCRs 
2x Phusion mix 25 ul
forward primer 2.5 ul
reverse primer 2.5 ul
DNA template (plasmid) 1 ul
H2O 19 ul
50 ul
  • R: No bands for fragments 3, Bands for fragment 4
  • C: Cut out bands of fragment 4, PCR again to obtain fragment 3


  • E: Measured concentration of DNA fragments 1 and 2
  • R: Concentrations okay
  • C: Do assembly PCR once enough of fragments 3 and 4 obtained
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