Team:Alberta/Project/AnchTermAnneal

From 2009.igem.org

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<h2>Procedure:</h2>
<h2>Procedure:</h2>
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To make a 10 μM Anchor solution with an A(B) overhang one must anneal Anchor_A(Anchor_B) and Anchor_Comp. To make a 10 μM Terminator solution with an A(B) overhang one must anneal Term_A(Term_B) and Term_Comp.

Revision as of 16:43, 21 October 2009

University of Alberta - BioBytes










































































































Annealing Anchor and Terminator Bytes

Instructions on how to anneal the complementary strands to obtain the same concentrations we used in our experiments (10μM).

What you will need:

  • Anchor oligos (complementary)
  • Terminator oligos (complementary)

Procedure:

To make a 10 μM Anchor solution with an A(B) overhang one must anneal Anchor_A(Anchor_B) and Anchor_Comp. To make a 10 μM Terminator solution with an A(B) overhang one must anneal Term_A(Term_B) and Term_Comp.

Retrieved from "http://2009.igem.org/Team:Alberta/Project/AnchTermAnneal"