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Team:NTU-Singapore/Notebook
From 2009.igem.org
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* Antibiotic stock preparation | * Antibiotic stock preparation | ||
* Inoculation | * Inoculation | ||
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| + | <u>'''20th June'''</u> | ||
| + | * Our first Plasmid Extraction (PE)! | ||
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| + | <u>'''22nd June'''</u> | ||
| + | * First Double Digestion (DD) and Gel Check! | ||
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| + | <u>'''23rd June '''</u> | ||
| + | * PE & DD of the registry constructs…. | ||
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| + | <u>'''24th June '''</u> | ||
| + | *Our direct synthesized parts finally arrived. We now have CHE (cholesterol esterase), IFP(Infrared protein 1.4) and pNO (NO sensing promoter). | ||
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| + | <u>'''26th June'''</u> | ||
| + | *PE , DD and Gel check for the direct synthesized gene sequence. | ||
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| + | <u>'''27th June '''</u> | ||
| + | *Gel Check for CHE, IFP and pNO | ||
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| + | ==July== | ||
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| + | <u>''' '''</u> | ||
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Revision as of 19:07, 21 October 2009
Experiment Timeline
June
18th June
- Preparation of LB medium
- Amplification of parts from registry (pTet, TetR, HO1, Low copy plasmid)
19th June
- Antibiotic stock preparation
- Inoculation
20th June
- Our first Plasmid Extraction (PE)!
22nd June
- First Double Digestion (DD) and Gel Check!
23rd June
- PE & DD of the registry constructs….
24th June
- Our direct synthesized parts finally arrived. We now have CHE (cholesterol esterase), IFP(Infrared protein 1.4) and pNO (NO sensing promoter).
26th June
- PE , DD and Gel check for the direct synthesized gene sequence.
27th June
- Gel Check for CHE, IFP and pNO
July
