Team:Heidelberg/Notebook measure/NotebookRT
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__NOTOC__ | __NOTOC__ | ||
+ | == '''Real-time RT-PCR''' == | ||
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== Contents == | == Contents == | ||
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! Week !! colspan="7" |Days | ! Week !! colspan="7" |Days | ||
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- | |style="text-align:center"| | + | |style="text-align:center"| 35 |
- | + | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#8-24-2009|8-24-2009]] | |
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+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#8-26-2009|8-26-2009]] | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#8-27-2009|8-27-2009]] | ||
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+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#8-31-2009|8-31-2009]] | ||
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- | |style="text-align:center"| [[Team:Heidelberg/ | + | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-02-2009|9-02-2009]] |
- | |style="text-align:center"| [[Team:Heidelberg/ | + | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-03-2009|9-03-2009]] |
- | |style="text-align:center"| [[Team:Heidelberg/ | + | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-04-2009|9-04-2009]] |
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- | + | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-10-2009|9-10-2009]] | |
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|style="text-align:center"| 39 | |style="text-align:center"| 39 | ||
- | |style="text-align:center"| [[Team:Heidelberg/ | + | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-21-2009|9-21-2009]] |
- | |style="text-align:center"| | + | |style="text-align:center"| - |
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- | |style="text-align:center"| [[Team:Heidelberg/ | + | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-24-2009|9-24-2009]] |
- | |style="text-align:center"| [[Team:Heidelberg/ | + | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-25-2009|9-25-2009]] |
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+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-30-2009|9-30-2009]] | ||
|style="text-align:center"| - | |style="text-align:center"| - | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#10-02-2009|10-02-2009]] | ||
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== 9-04-2009== | == 9-04-2009== | ||
*RNA extration of HeLa transfected with CMV and JeT. | *RNA extration of HeLa transfected with CMV and JeT. | ||
+ | **yield: 3.7 µg/1.5*10<sup>5</sup> cells | ||
+ | [[Image:HD09_RNA_GEL1.png|thumb|left|900px|<div style="text-align:justify;">'''Gel electrophoresis of RNA extracted from HeLa cells.''' 1% agarose gel, 100V, 60 min. </div>]] | ||
*Real-time RT PCR | *Real-time RT PCR | ||
- | **result:mRNA(CMV):mRNA(JeT)=1. | + | **result:mRNA(CMV):mRNA(JeT)=1.73:1 |
== 9-10-2009== | == 9-10-2009== | ||
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*Dilution: to 20 ng/µl | *Dilution: to 20 ng/µl | ||
*gel electrophoresis: positive | *gel electrophoresis: positive | ||
+ | [[Image:HD09_RNA_GEL2.png|thumb|left|900px|<div style="text-align:justify;">'''Gel electrophoresis of RNA extracted from HeLa cells.''' 1% agarose gel, 100V, 60 min. </div>]] | ||
== 9-25-2009== | == 9-25-2009== | ||
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**Dilute the products to 20 ng/µl | **Dilute the products to 20 ng/µl | ||
*Real-time RT PCR for CMV and JeT (50h) | *Real-time RT PCR for CMV and JeT (50h) | ||
+ | |||
+ | |} |
Latest revision as of 19:24, 21 October 2009
Real-time RT-PCRContents
8-24-2009
8-26-2009
8-31-2009
9-02-2009
9-03-2009
9-04-2009
9-10-2009
9-21-2009
9-24-2009
9-25-2009
9-30-2009
10-02-2009
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