Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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== October 21, 2009 == | == October 21, 2009 == | ||
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+ | The IPTG preparation protocol we used is discribed [http://openwetware.org/wiki/IPTG HERE!!!] | ||
For the induction of protein production again we start our culture at .1 abs 620nm and wait until it reached | For the induction of protein production again we start our culture at .1 abs 620nm and wait until it reached | ||
- | .35 abs 620 nm then we added IPTG to a final concentration of .1mM and then we leave the culture media for four | + | 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four |
- | hours to get some expression from the plasmid that have our | + | hours to get some expression from the plasmid that have our construction. |
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Revision as of 19:31, 21 October 2009