Team:Alberta/Project/Gel Extraction

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<li>Eppendorf tube
<li>Eppendorf tube
<li>Gel extraction kit
<li>Gel extraction kit
-
<li>55oC water bath
+
<li>55degC water bath
<li>Vortex
<li>Vortex
<li>Microfuge  
<li>Microfuge  

Revision as of 19:44, 21 October 2009

University of Alberta - BioBytes










































































































Gel Extraction

What you will need:

  • Clean scalpel
  • Transilluminator
  • Eppendorf tube
  • Gel extraction kit
  • 55degC water bath
  • Vortex
  • Microfuge

Procedure

  • Place the gel on the transilluminator. Put on face shield and gloves.
  • urn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
  • Pry out the gel ‘cube’ and place it in an eppendorf tube.
  • Follow instructions in kit manual.

Retrieved from "http://2009.igem.org/Team:Alberta/Project/Gel_Extraction"