Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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The IPTG preparation protocol we used is discribed [http://openwetware.org/wiki/IPTG HERE!!!] | The IPTG preparation protocol we used is discribed [http://openwetware.org/wiki/IPTG HERE!!!] | ||
- | For the induction of protein production again we | + | For the induction of protein production again we started our culture at .1 abs 620nm in 100mL LB and waited until it reached 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four |
- | 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four | + | hours to see if the GFP that is under the control of the multipromotr is expressed. |
- | hours to | + | |
+ | Using microscope with suitable filters and light to see GFP we got the following pictures form the IPTG induced BL21 strain that contained our construction, the BL21 with our construction but without IPTG inductor; BL21 that doesn't have | ||
+ | hour construction didn't fluoresce at all so this starts to support functioning of the multipromoter. | ||
Revision as of 19:44, 21 October 2009