Team:Alberta/Project/Gel Extraction

From 2009.igem.org

(Difference between revisions)
(New page: {{:Team:Alberta/Template3}} <html> <head> <style type="text/css"> .b1f, .b2f, .b3f, .b4f{font-size:1px; overflow:hidden; display:block;} .b1f {height:1px; background:#e1e1e1; margin:0 5px;...)
 
(One intermediate revision not shown)
Line 34: Line 34:
<li>Eppendorf tube
<li>Eppendorf tube
<li>Gel extraction kit
<li>Gel extraction kit
-
<li>55oC water bath
+
<li>55degC water bath
<li>Vortex
<li>Vortex
<li>Microfuge  
<li>Microfuge  
Line 43: Line 43:
<ul>
<ul>
     <li>Place the gel on the transilluminator. Put on face shield and gloves.
     <li>Place the gel on the transilluminator. Put on face shield and gloves.
-
     <li>urn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
+
     <li>Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
     <li>Pry out the gel ‘cube’ and place it in an eppendorf tube.
     <li>Pry out the gel ‘cube’ and place it in an eppendorf tube.
     <li>Follow instructions in kit manual.  
     <li>Follow instructions in kit manual.  

Latest revision as of 19:44, 21 October 2009

University of Alberta - BioBytes










































































































Gel Extraction

What you will need:

  • Clean scalpel
  • Transilluminator
  • Eppendorf tube
  • Gel extraction kit
  • 55degC water bath
  • Vortex
  • Microfuge

Procedure

  • Place the gel on the transilluminator. Put on face shield and gloves.
  • Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
  • Pry out the gel ‘cube’ and place it in an eppendorf tube.
  • Follow instructions in kit manual.

Retrieved from "http://2009.igem.org/Team:Alberta/Project/Gel_Extraction"