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Team:British Columbia/pBAD
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=Arabinose sensor: the pBAD promoter= | =Arabinose sensor: the pBAD promoter= | ||
==Overview== | ==Overview== | ||
| - | In order to make an analog biosensor, we need our traffic light to be able to respond differently to | + | In order to make an analog biosensor, we need our traffic light to be able to respond differently to various concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the <partinfo>I13453</partinfo> pBAD promoter, one weaker and one stronger, based on AraC binding experiments performed by [[Team:British_Columbia/Bibliography|Niland et al.]] |
[[Image:E_coli_Traffic_Light_Mutated_Promoters.png|left|thumb||500px|A diagram of the changed nucleotide sequences of arabinose-inducible promoters. Green nucleotides of the wildtype show all sequences that were mutated to form strong and weak.]] | [[Image:E_coli_Traffic_Light_Mutated_Promoters.png|left|thumb||500px|A diagram of the changed nucleotide sequences of arabinose-inducible promoters. Green nucleotides of the wildtype show all sequences that were mutated to form strong and weak.]] | ||
Revision as of 19:44, 21 October 2009
Home Team Traffic Light Sensor Lock&Key Jammer [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=British_Columbia Parts] Safety Sponsors Notebook Bibliography
Arabinose sensor: the pBAD promoter
Overview
In order to make an analog biosensor, we need our traffic light to be able to respond differently to various concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the pBAD promoter, one weaker and one stronger, based on AraC binding experiments performed by Niland et al.
Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at (strong) and (weak).
Using these promoter sequences, we assembled XXX...
