Team:Kyoto/CiC/Parts

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Revision as of 19:48, 21 October 2009

Parts

Part design

In this project, we designed two plasmid for RTS; 1. HIV-TAT::(LALAAAA)3 expressing vector 2. Signal for TIM23 complex::GFP expressing vector 3.Signal for TIM23 complex::EGFP expressing vector for HeLa cells. Both of 1 and 3 have T7 promoter, terminator and each CDS between them. They can express each protein by RTS (Rapidly Translation System, Roache). Both parts are inserted in high copy number plasmid pSB1A2. 3.Signal for TIM23 complex::EGFP expressing vector for HeLa cells is made by inserting Signal for TIM23 complex into pEGFP-N3.

HIV-TAT-(LALAAAA)3-generator

TIM23complex signal::EGFP generator

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 ==Parts==
 ===Part design===
-In this project, we designed two plasmid for RTS; 1. HIV-TAT::(LALAAAA)3 expressing vector 2. Signal for TIM23 complex::GFP expressing vector. Both of them have T7 promoter, terminator and each CDS between them. They can express each protein by RTS (Rapidly Translation System, Roache). Both parts are inserted in high copy number plasmid pSB1A2.
+In this project, we designed two plasmid for RTS; 1. HIV-TAT::(LALAAAA)3 expressing vector 2. Signal for TIM23 complex::GFP expressing vector 3.Signal for TIM23 complex::EGFP expressing vector for HeLa cells. Both of 1 and 3 have T7 promoter, terminator and each CDS between them. They can express each protein by RTS (Rapidly Translation System, Roache). Both parts are inserted in high copy number plasmid pSB1A2. 3.Signal for TIM23 complex::EGFP expressing vector for HeLa cells is made by inserting Signal for TIM23 complex into pEGFP-N3.
 ===HIV-TAT-(LALAAAA)3-generator===