Team:ArtScienceBangalore/Notebook/Week Four

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(New page: ===June 8th- 17th=== '''What are we trying to do?''' We want the bacteria to lyse, to kill itself from the inside. Well then, to do that we had to get a lysis gene in it. The thing is, ...)
 
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      <big><h1>Week Four</h1></big>
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===June 8th- 17th===
===June 8th- 17th===
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How we tried to do this:
How we tried to do this:
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'''[[Step 1 - Taking dry DNA from wells]]'''
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'''[http://hackteria.org/wiki/index.php/Step_1_-_Taking_dry_DNA_from_wells Step 1 - Taking dry DNA from wells]'''
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'''[[Step 2 - Transforming competent cells]]'''
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'''[http://hackteria.org/wiki/index.php/Step_2_-_Transforming_competent_cells Step 2 - Transforming competent cells]'''
'''Step 3 - Picking a single colony.'''
'''Step 3 - Picking a single colony.'''
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'''Step 4 - Inoculating broth with Ampicillin-R''' (an antibiotic) and letting it grow for 18 hours.
'''Step 4 - Inoculating broth with Ampicillin-R''' (an antibiotic) and letting it grow for 18 hours.
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'''Step 5 - Using the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.''
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'''Step 5 - Using the resulting culture for [http://hackteria.org/wiki/index.php/Mini-prep. mini-prep.]''' - ''a process used to purify plasmids and yields clean, usable DNA.''
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'''Step 6 - [[Digesting the DNA]]'''
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'''Step 6 - [http://hackteria.org/wiki/index.php/Digesting_the_DNA Digesting the DNA]'''
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'''Step 7 - [[Gel Electrophoresis]]'''
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'''Step 7 - [http://hackteria.org/wiki/index.php/Gel_Electrophoresis Gel Electrophoresis]'''
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'''Step 8-  [[Ligation]]'''
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'''Step 8-  [http://hackteria.org/wiki/index.php/Ligation Ligation]'''
'''Step 9-  [[Transformation and Inoculation]]'''
'''Step 9-  [[Transformation and Inoculation]]'''
===June 9th===
===June 9th===
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[[The June 9th Image Gallery]]
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[http://hackteria.org/wiki/index.php/The_June_9th_Image_Gallery The June 9th Image Gallery]
Images of the various results attained:
Images of the various results attained:
<gallery>
<gallery>
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File:Gelimage090609.png|Agarose gel electrophoresis image of digested K112808
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Image:Gelimage090609.png|Agarose gel electrophoresis image of digested K112808
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File:Not working.jpg|Failure 1
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Image:Not working.jpg|Failure 1
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File:PBAD-insert f.png| The pBAD Insert
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Image:PBAD-insert f.png| The pBAD Insert
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File:Failed Ligation.png| The Failed Ligation Attempt
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Image:Failed Ligation.png| The Failed Ligation Attempt
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Latest revision as of 21:20, 21 October 2009

Week Four

June 8th- 17th

What are we trying to do?

We want the bacteria to lyse, to kill itself from the inside.

Well then, to do that we had to get a lysis gene in it. The thing is, with the lysis gene needs to be turned on by this specific substance called a promoter

There are two kinds of promoters, ones that make the gene they're attached to , be 'on' all the time. Then, you have ones that turn on when you want them to, in our case, when you add a mystery enzyme.

Our Step-wise Process:

How we tried to do this:

[http://hackteria.org/wiki/index.php/Step_1_-_Taking_dry_DNA_from_wells Step 1 - Taking dry DNA from wells]

[http://hackteria.org/wiki/index.php/Step_2_-_Transforming_competent_cells Step 2 - Transforming competent cells]

Step 3 - Picking a single colony.

Step 4 - Inoculating broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.

Step 5 - Using the resulting culture for [http://hackteria.org/wiki/index.php/Mini-prep. mini-prep.] - a process used to purify plasmids and yields clean, usable DNA.

Step 6 - [http://hackteria.org/wiki/index.php/Digesting_the_DNA Digesting the DNA]

Step 7 - [http://hackteria.org/wiki/index.php/Gel_Electrophoresis Gel Electrophoresis]

Step 8- [http://hackteria.org/wiki/index.php/Ligation Ligation]

Step 9- Transformation and Inoculation

June 9th

[http://hackteria.org/wiki/index.php/The_June_9th_Image_Gallery The June 9th Image Gallery]

Images of the various results attained:

Agarose gel electrophoresis image of digested K112808

Failure 1

The pBAD Insert

The Failed Ligation Attempt

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