Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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early lysis that occur when WT P4 and P2 interact. This avoidance will be achieved by taking the control of the | early lysis that occur when WT P4 and P2 interact. This avoidance will be achieved by taking the control of the | ||
two major regulators of P2 morphopoietic genes. The control systems that is going to be implemented is constituted | two major regulators of P2 morphopoietic genes. The control systems that is going to be implemented is constituted | ||
- | by a promoter inducible by IPTG | + | by a promoter inducible by IPTG in conjunction with transactivators cox and ogr from phage P2. All this will |
- | allow us to grow bacteria in grate quantities and induce lysis when ever we want and as a consequence the | + | allow us to grow bacteria in grate quantities and induce lysis when ever we want and as a consequence the |
- | of grate amounts of P4 phage. | + | production of grate amounts of P4 phage. |
Qualitative characterization of the multipromoter. | Qualitative characterization of the multipromoter. | ||
- | The multipromoter that we have designed has the capacity to | + | The multipromoter that we have designed has the capacity to respond specifically to T3/T7 RNA polymerases |
+ | so if one or both polymarease are presente in the cell the genes downstream of this promoter will be active. | ||
+ | A first characterization approach is the introduction of a plasmid carring our promoter in the E. coli strain | ||
+ | BL21(DE3)pLysS that has a T7 RNA polymerase inducible by IPTG | ||
== July 30, 2009 == | == July 30, 2009 == |
Revision as of 21:59, 21 October 2009