Team:IIT Bombay India/Notebook

From 2009.igem.org

(Difference between revisions)

Latest revision as of 22:01, 21 October 2009

Home	The Team	The Project	Analysis	Modeling	Notebook	Safety

Week1 -FACS Trial

Week2 - (May, 18-23) Preparation

Week3 - (May, 25-30) FACS

Week4 - (Jun, 1-6) Growth Curve

Week5 - (Jun, 8-13) FACS:YFP signal

Week6 - (Jun, 15-20) FACS:CFP signal

Week7 - (Jun, 22-27) FACS repeat

Week8 - (Jun-Jul, 29-4) Req.:Transformation

Week9 - (Jul, 6-11) Cultured Host

Week10- (Jul, 13-18) Transformation of 4 strains

Week11- (Jul, 20-24) FACS:Constructs

Week12- (Jul, 27-31) FACS

Week13- (Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)

Week14- (Aug, 10-15) Confirmation of the transformants

Week15- (Aug, 17-22) Preparation for time course expression analysis

Week16- (Aug, 24-29) FACS time course

Week17- (Aug-Sep, 31-5) FACS: different IPTG conc.

Week18- (Sep, 7-13) Growth curves for all the four strains

Week19- (Sep, 14-20) Growth curve on Lactose (ptet A and plac K)

Week20- (Sep, 21-27) Growth curve on Lactose (plac A and ptet K)

Week21- (Sep-Oct, 28-3) Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)

Week22- (Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)

Week23- (Oct, 12-17) Growth curve on Lactose (ptet A and plac K)

Week24- (Oct, 18-21) Data compilation and analysis, wiki uploads

@@ Line 1: / Line 1: @@
-[[Week1]] FACS Trial (May, 18-23)
+{| style="color:#000000;background-color:#ffffff;" cellpadding="6" cellspacing="3" border="0" bordercolor="#ffffff" width="65%" align="center"
-[[Week2]] Preparation (May, 25-30)
+!align="center"|[[Team:IIT_Bombay_India|Home]]
+!align="center"|[[Team:IIT_Bombay_India/Team|The Team]]
+!align="center"|[[Team:IIT_Bombay_India/Project|The Project]]
+!align="center"|[[Team:IIT_Bombay_India/Analysis|Analysis]]
+!align="center"|[[Team:IIT_Bombay_India/Modeling|Modeling]]
+!align="center"|[[Team:IIT_Bombay_India/Notebook|Notebook]]
+!align="center"|[[Team:IIT_Bombay_India/Safety|Safety]]
+|}
-[[Week3]] FACS (Jun, 1-6)
-[[Week4]] Growth Curve (Jun, 8-13)
-[[Week5]] FACS:YFP signal (Jun, 15-20)
+[[Image:IITB-Home.jpg]]
-[[Week6]] FACS:CFP signal (Jun, 22-27)
+{| background-color:#ffffff;" cellpadding="1.5" cellspacing="2" border="0" bordercolor="#ffffff" width="90%" align="center"
+!align="left"|
-[[Week7]] FACS repeat (Jun 29- Jul 4)
+|
-[[Week8]] Req.:Transformation (Jul, 6-11)
-[[Week9]] Cultured Host (Jul, 13-18)
+[[Week1]] -FACS Trial
-[[Week10]] Transformation of 4 strains (Jul, 20-24)
+[[Week2]] - (May, 18-23)     Preparation
-[[Week11]] FACS :Constructs (Jul, 27-31)
+[[Week3]] - (May, 25-30)     FACS
-[[week 12]] FACS
+[[Week4]] - (Jun, 1-6)       Growth Curve
+[[Week5]] - (Jun, 8-13)      FACS:YFP signal
+[[Week6]] - (Jun, 15-20)     FACS:CFP signal
+[[Week7]] - (Jun, 22-27)     FACS repeat
+[[Week8]] - (Jun-Jul, 29-4)  Req.:Transformation
+[[Week9]] - (Jul, 6-11)      Cultured Host
+[[Week10]]- (Jul, 13-18)     Transformation of 4 strains
+[[Week11]]- (Jul, 20-24)     FACS:Constructs
+[[Week12]]- (Jul, 27-31)     FACS
+[[Week13]]- (Aug, 3-8)       Transformations in ''E.coli'' MG1655 (lac I deleted)
+[[Week14]]- (Aug, 10-15)     Confirmation of the transformants
+[[Week15]]- (Aug, 17-22)     Preparation for time course expression analysis
+[[Week16]]- (Aug, 24-29)     FACS time course
+[[Week17]]- (Aug-Sep, 31-5)  FACS: different IPTG conc.
+[[Week18]]- (Sep, 7-13)      Growth curves for all the four strains
+[[Week19]]- (Sep, 14-20)     Growth curve on Lactose (ptet A and plac K)
+[[Week20]]- (Sep, 21-27)     Growth curve on Lactose (plac A and ptet K)
+[[Week21]]- (Sep-Oct, 28-3)  Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)
+[[Week22]]- (Oct, 5-10)      Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)
+[[Week23]]- (Oct, 12-17)     Growth curve on Lactose (ptet A and plac K)
+[[Week24]]- (Oct, 18-21)     Data compilation and analysis, wiki uploads