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Team:UAB-Barcelona/Primersn
From 2009.igem.org
(Difference between revisions)
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Reverse: | Reverse: | ||
S | S | ||
| - | GTCAACGT ACTAGT CTGCACGAAAGACCTTTAAATAC | + | GTCAACGT ACTAGT CTGCACGAAAGACCTTTAAATAC Annealing temperature = 62 ºC |
Forward: | Forward: | ||
E N X | E N X | ||
| - | CAGTAGTA GAATTC GCGGCCGC TCTAGA GTTGCGCGAAATACCGTAAC | + | CAGTAGTA GAATTC GCGGCCGC TCTAGA GTTGCGCGAAATACCGTAAC Annealing temperature= 60 ºC |
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| + | |||
| + | |||
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| + | • nsrR: X – nsrR – S – N- P | ||
| + | |||
| + | Reverse: | ||
| + | X RBS Linker | ||
| + | GTCAACGT TCTAGA TAAGGAGG AATATAA ATGAGACTGACGAATTACAGCGATTAC Annealing temperature = 76 ºC | ||
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| + | Forward: | ||
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| + | P N S | ||
| + | CAGTAGTA CTGCAG GCGGCCGC ACTAGT GCCAAGCTGACCTACAGTTTATTCC Annealing temperature = 76 ºC | ||
Latest revision as of 22:05, 21 October 2009
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These were the primers we ordered for the nitrite: • Pnir: EcoR1-Not-Xba— nirk – Spe Reverse: S
GTCAACGT ACTAGT CTGCACGAAAGACCTTTAAATAC Annealing temperature = 62 ºC
Forward: E N X
CAGTAGTA GAATTC GCGGCCGC TCTAGA GTTGCGCGAAATACCGTAAC Annealing temperature= 60 ºC
Reverse: X RBS Linker
GTCAACGT TCTAGA TAAGGAGG AATATAA ATGAGACTGACGAATTACAGCGATTAC Annealing temperature = 76 ºC
P N S
CAGTAGTA CTGCAG GCGGCCGC ACTAGT GCCAAGCTGACCTACAGTTTATTCC Annealing temperature = 76 ºC
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