Team:UAB-Barcelona/Primersp

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These were the primers we ordered for the nitrite:
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These were the primers we ordered for the phosphate:
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• Pnir: EcoR1-Not-Xba— nirk – Spe
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• phoA: EcoR1-Not-Xba— phoA – Spe
Reverse:
Reverse:
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                S
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                  S
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     GTCAACGT ACTAGT CTGCACGAAAGACCTTTAAATAC    Annealing temperature = 62 ºC
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     5’ GCTCATA ACTAGT TTTATTTTCTCCATGTACAAATACATTAAAA 3’ Annealing temperature = 74 ºC
Forward:  
Forward:  
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                  E      N      X
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                    E      N      X
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       CAGTAGTA GAATTC GCGGCCGC TCTAGA GTTGCGCGAAATACCGTAAC   Annealing temperature= 60 ºC
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       5’ AGATTCA GAATTC GCGGCCGC TCTAGA GTTTCAACAGCTGTCATAAAGTTGTCAC  3’   Annealing temperature= 74 ºC
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• nsrR: X – nsrR – S – N- P
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Reverse:
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                  X    RBS    Linker
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      GTCAACGT TCTAGA TAAGGAGG AATATAA ATGAGACTGACGAATTACAGCGATTAC  Annealing temperature = 76 ºC
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Forward:
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                  P      N      S
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      CAGTAGTA CTGCAG GCGGCCGC ACTAGT GCCAAGCTGACCTACAGTTTATTCC      Annealing temperature = 76 ºC
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Latest revision as of 23:04, 21 October 2009

Encap raro.jpg Documento sin título

These were the primers we ordered for the phosphate:

• phoA: EcoR1-Not-Xba— phoA – Spe

Reverse: 

                 S
    5’ GCTCATA ACTAGT TTTATTTTCTCCATGTACAAATACATTAAAA 3’ Annealing temperature = 74 ºC

Forward: 

                    E      N       X
     5’ AGATTCA GAATTC GCGGCCGC TCTAGA GTTTCAACAGCTGTCATAAAGTTGTCAC  3’   Annealing temperature= 74 ºC
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