Team:UAB-Barcelona/PCRP

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'''Annealing temperature'''
'''Annealing temperature'''
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nirk (270 pb): as lowest temperature is 60ºC, the temperature we should set in the thermociclator would be 55ºC. But it didn't work properly at this temperature, so we set the temperature to 52ºC and 49ºC. PCR in both cases worked properly
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''phoA'' (100 pb): as annealing temperature is 74ºC, the temperature we should set in the thermociclator would be 69ºC. But it didn't work properly at this temperature, so we set the temperature to 66ºC and 64ºC. PCR in both cases worked properly
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https://static.igem.org/mediawiki/2009/1/12/NirK.jpg
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Marker, 3x49ºC, controls, marker, 3x52ºC, controls
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https://static.igem.org/mediawiki/2009/3/37/PhoA.jpg
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'''Annealing temperature'''
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nsr(500 pb): the temperature is 76ºC, so we made the PCR at 71ºC. It didn't work. But when we tried with 65 and 68ºC it worked properly.
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https://static.igem.org/mediawiki/2009/f/f3/Pnsr.jpg
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Marker, 4x65ºC, controls, marker, 4x68ºC, controls
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Revision as of 23:55, 21 October 2009

Encap raro.jpg Documento sin título

PCR of nitrite detection sequences

We used GoTaq Green Master Mix (Promega)12

Annealing temperature

phoA (100 pb): as annealing temperature is 74ºC, the temperature we should set in the thermociclator would be 69ºC. But it didn't work properly at this temperature, so we set the temperature to 66ºC and 64ºC. PCR in both cases worked properly

PhoA.jpg

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