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Team:British Columbia/pBAD
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Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at <partinfo>K206000</partinfo> (strong) and <partinfo>K206001</partinfo> (weak). | Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at <partinfo>K206000</partinfo> (strong) and <partinfo>K206001</partinfo> (weak). | ||
| - | <html>We assembled each promoter with the RFP reporter part | + | <html>We assembled each promoter with the RFP reporter part <partinfo>I13507</partinfo> in order to test the relative activity of the mutated promoters</html> |
[[Image:Timecourse.jpg]] | [[Image:Timecourse.jpg]] | ||
[[Image:Toxicity.jpg]] | [[Image:Toxicity.jpg]] | ||
[[Image:Trans_fcn.jpg]] | [[Image:Trans_fcn.jpg]] | ||
Revision as of 00:14, 22 October 2009
Home Team Traffic Light Sensor Lock&Key Jammer [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=British_Columbia Parts] Safety Sponsors Notebook Bibliography
Arabinose sensor: the pBAD promoter
Overview
In order to make an analog biosensor, we need our traffic light to produce distinct, unique responses to a range of concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the pBAD promoter, one weaker and one stronger than wild type, based on AraC binding experiments performed by Niland et al.
Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at (strong) and (weak).
We assembled each promoter with the RFP reporter part
