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Team:UAB-Barcelona/CS1
From 2009.igem.org
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''Biobrick 11'' + ''Biobrick 5'' // Biobrick 13 | ''Biobrick 11'' + ''Biobrick 5'' // Biobrick 13 | ||
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''Biobrick 11'' + ''Biobrick 6 '' // Biobrick 14 | ''Biobrick 11'' + ''Biobrick 6 '' // Biobrick 14 | ||
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''Biobrick 9'' (X,P) + '''RBS-GFP-T-T''' BBa_I13504 (S,P) // Biobrick 15 | ''Biobrick 9'' (X,P) + '''RBS-GFP-T-T''' BBa_I13504 (S,P) // Biobrick 15 | ||
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''Biobrick 10'' (X,P) + '''RBS-GFP-T-T''' BBa_I13504 (S,P) // Biobrick 16 | ''Biobrick 10'' (X,P) + '''RBS-GFP-T-T''' BBa_I13504 (S,P) // Biobrick 16 | ||
Revision as of 00:33, 22 October 2009
Contents |
Cloning strategy
1st round
phoa (E,S) + backbone BBa_K093005 (E,S) // Biobrick 1
nirk (E,S) + backbone BBa_K093005 (E,S) // Biobrick 2
nsr (X,P) + backbone BBa_K093005 (E,S) // Biobrick 3
phoa (E,S) + RBS-LacI BBa_J24679 (E,X) // Biobrick 4
pLacI-RBS-RFP-T-T BBa_J04450 (E,S) + linker BBa_B0040 (E,X) // Biobrick 5
ptet-RBS-RFP-T-T BBa_I13521 (E,S) + linker BBa_B0040 (E,X) // Biobrick 6
nirk (E,S) + RBS-LacI BBa_J24679 (E,X) // Biobrick 7
RBS BBa_J61127 (S,P) + nsr (X,P) // Biobrick 8
ptet BBa_R0040 (S,P) + Biobrick 8 (X,P) // Biobrick 9
placI BBa_K091112 (S,P) + Biobrick 8 (X,P) // Biobrick 10
2nd round
Biobrick 4 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 11
Biobrick 7 (X,P) + RBS-GFP-T-T BBa_E0840 (S,P) // Biobrick 12
3rd round
Biobrick 11 + Biobrick 5 // Biobrick 13
Biobrick 11 + Biobrick 6 // Biobrick 14
Biobrick 9 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 15
Biobrick 10 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 16
