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| - | ==Project Notebook==
| + | {{Template:UAB-Barcelona2}} |
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| | + | =Project Notebook= |
| | <html> | | <html> |
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| - | table.calendar { margin: 0; padding: 10px; } | + | table.calendar { margin: 0px; padding: 10px; } |
| - | table.calendar td { margin: 0; padding: 2px; vertical-align: top; } | + | table.calendar td { margin: 0px; padding: 2px; vertical-align: top; } |
| - | table.month .heading td { padding:2px; background-color:#d4d4d4; color:#aaa; text-align:center; font-size:120%; font-weight:bold; } | + | table.month .heading td { padding: 2px; background-color:#d4d4d4; color:#aaa; text-align:center; font-size:120%; font-weight:bold; } |
| | table.month .dow td { color:#aaa; text-align:center; font-size:110%; } | | table.month .dow td { color:#aaa; text-align:center; font-size:110%; } |
| | table.month td.today { background-color:#ddd; } | | table.month td.today { background-color:#ddd; } |
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| | font-weight: bold; | | font-weight: bold; |
| | font-size: 8pt; | | font-size: 8pt; |
| - | text-align: right; | + | text-align: center; |
| | background-color: #eee; | | background-color: #eee; |
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| | |} | | |} |
| | | | |
| - | ''' Wednesday 8th July'''
| + | =PROTOCOLS USED= |
| | + | {|class="wikitable" border="0" cellpadding="10" cellspacing="1" style="padding: 0; background-color:black; border: 1px solid black; text-align:justify; margin:5px -5px 5px -5px" |
| | + | !width="30%"; valign="top" style="background:#ECC850; color:black"| |
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| - | Looking for sponsors near Barcelona.
| + | If you want to check which methods we used during this summer, we are pleased to show you which Protocols were followed to do the most part of the experimental work. Among of them you will find: |
| | | | |
| - | Reading and discussing the proyect’s academic records.
| + | -How to prepare a medium to make ''Nitrosomonas Europaea'' grow. |
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| - |
| + | -How to purify genomic DNA. |
| - | ''' Thursday 9th July'''
| + | |
| | | | |
| - | Looking for sponsors near Barcelona.
| + | -How to do an agarose gel electrophoresis. |
| | | | |
| - | Looking for several suitable mediums for Nitrosomonas europaea culture. The following articles were consulted and discussed, besides ATCC medium:
| + | -How to prepare competent cells. |
| | | | |
| - | - Test medium for the growth of Nitrosomonas europaea; Chikashi Sato, Jerald L. Schnoor, Donald B. McDonald and Jon Huey; Applied and enviromental microbiology, May 1985, Vol. 49, No. 5, p. 1101-1107. | + | -Etc... |
| | | | |
| - | - Improved methods for the cultivation of the chemolithoautotrofic bacterium Nitrosomonas europaea; Bernice D. Chapman, Martina Scheleicher, Abraham Beuger, Peter Gotomski and Jürgen H. Thiele; Journal of Microbiological methods 65 2006 96-106.
| + | [[Media:Protocols.pdf|PROTOCOLS]] |
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| - | - Ammonia or ammonium ion as substrate for oxidation by Nitrosomonas europaea cells and extracts. Isamu Suzuki, Usha Dular and S.C. Kwok; Journal of Bacteriology, Oct. 1974 Vol. 120, No. 1 p. 556-558. | + | |- |
| - | | + | |} |
| - | | + | |
| - | ''' Friday 10th July'''
| + | |
| - | | + | |
| - | Looking for sponsors from Andalucia’s bioregion.
| + | |
| - | | + | |
| - | Looking for several suitable mediums for Nitrosomonas europaea culture. The following articles were consulted and discussed, besides ATCC medium:
| + | |
| - | | + | |
| - | - Test medium for the growth of Nitrosomonas europaea; Chikashi Sato, Jerald L. Schnoor, Donald B. McDonald and Jon Huey; Applied and enviromental microbiology, May 1985, Vol. 49, No. 5, p. 1101-1107.
| + | |
| - | | + | |
| - | - Improved methods for the cultivation of the chemolithoautotrofic bacterium Nitrosomonas europaea; Bernice D. Chapman, Martina Scheleicher, Abraham Beuger, Peter Gotomski and Jürgen H. Thiele; Journal of Microbiological methods 65 2006 96-106.
| + | |
| - | | + | |
| - | - Ammonia or ammonium ion as substrate for oxidation by Nitrosomonas europaea cells and extracts. Isamu Suzuki, Usha Dular and S.C. Kwok; Journal of Bacteriology, Oct. 1974 Vol. 120, No. 1 p. 556-558.
| + | |
| - | | + | |
| - | Looking for possible metabolic ways in order to be ahead of strange experiment results. The following article was discussed:
| + | |
| - | | + | |
| - | - Global metabolite analisis: the influence of extraction metodology on metabolome profiles of Escherichia coli; Ram Prasam Maharjan and Thomas Ferenci; Analitical biochemistry 313 2003 145-154.
| + | |
| - | | + | |
| - | | + | |
| - | ''' Saturday 11th July'''
| + | |
| - | | + | |
| - | Looking for possible metabolic ways in order to be ahead of strange experiment results. The following article was discussed:
| + | |
| - | | + | |
| - | - A mechanistic perspective on bacterial metabolism of chlorinated methanes; Lawrence p. Wackett, Michael S.P. Logan, Frank A. Blocki and Cai Bao-li; Biodegradation 3 1992 19-32.
| + | |
| - | | + | |
| - | | + | |
| - | ''' Sunday 12th July'''
| + | |
| - | | + | |
| - | Looking for possible metabolic ways in order to be ahead of strange experiment results. The following article was discussed:
| + | |
| - | | + | |
| - | - A mechanistic perspective on bacterial metabolism of chlorinated methanes; Lawrence p. Wackett, Michael S.P. Logan, Frank A. Blocki and Cai Bao-li; Biodegradation 3 1992 19-32. Applied and enviromental microbiology, Nov. 1990, Vol. 56, No. 11, p. 3247-3254.
| + | |
| - | | + | |
| - | | + | |
| - | ''' Monday 13th July'''
| + | |
| - | | + | |
| - | Looking for sponsors from Andalucia’s bioregion.
| + | |
| - | | + | |
| - | Searching papers about E.coli metabolism.
| + | |
| - | | + | |
| - | | + | |
| - | ''' Tuesday 14th July'''
| + | |
| - | | + | |
| - | Looking for sponsors from Andalucia’s bioregion.
| + | |
| - | | + | |
| - | Searching papers about E.coli metabolism.
| + | |
| - | | + | |
| - | iGEM team meeting.
| + | |
| - | | + | |
| - | | + | |
| - | ''' Wednesday 15th July'''
| + | |
| - | | + | |
| - | Looking for sponsors from Andalucia’s bioregion.
| + | |
| - | | + | |
| - | Transformation of E.coli competent cells, in two different samples, with mlba and clpb GFP plasmids, using a thermoshock protocol.
| + | |
| - | | + | |
| - | Looking for possible metabolic ways in order to be ahead of strange experiment results. The following article was discussed:
| + | |
| - | | + | |
| - | - Reductive dehalogenation of carbon tetrachloride by Escherichira coli K-12; Craig S. Criddle, John T. DeWitt and Perry L. McCarty; Applied and enviromental microbiology, Nov 1990, Vol. 56, No. 11, p. 3247-3254.
| + | |
| - | | + | |
| - | The following paper was found and disccused, in order to find out if energetic metabolism could be affected:
| + | |
| - | | + | |
| - | - The effect of chloroform on mitochondrial energy transduction; Lee-Feng Chien and Martin D. Brand; Biochem. J.1996 320 837-845.
| + | |
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