Team:DTU Denmark/notebook

From 2009.igem.org

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     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark" >Home</a></font> </td>
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark" >Home</a></font> </td>
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     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/team" >The Team</a> </font></td>
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     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/team">The Team</a> </font></td>
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     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/project" >The Project</a> </font></td>
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     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/project">The Project</a> </font></td>
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     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/parts" >Parts submitted</a> </font></td>
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     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/parts">Parts submitted</a> </font></td>
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/modelling">Modelling</a></font> </td>
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/modelling">Modelling</a></font> </td>
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     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/notebook" title="Day to day lab activity">Notebook</a>  
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     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/notebook">Notebook</a>  
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     Also check out our Danish website<br><br>
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<br>   
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     Activities relating to our two sub-projects:<br><br>
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     <a href="http://www.bio.dtu.dk/igem.aspx" CLASS=leftbar>DTU iGEM 2009</a><br>
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     <a href="https://2009.igem.org/Team:DTU_Denmark/notebookredoxilator" CLASS=leftbar>- The Redoxilator</a><br>
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     <br>
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    <a href="https://2009.igem.org/Team:DTU_Denmark/notebookuserfusion" CLASS=leftbar>- The USER assembly standard</a><br>
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    <a href="https://2009.igem.org/Team:DTU_Denmark/notebookbiobrick" CLASS=leftbar>- Biobricks</a><br>
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     <a href="https://2009.igem.org/Team:DTU_Denmark/protocols" CLASS=leftbar>- Protocols</a><br>
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  <br>
   <b>Day-to-day activities</b><br><br><br>
   <b>Day-to-day activities</b><br><br><br>
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<font size="4"><b>Project abstract</b></font><br><br>
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In this section we have described our daily activities. The description of our experimental work is divided into the following sections:<br>
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*The activities related to the design, construction and testing of the Redoxilator.<br>
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*The events leading to the USER assembly standard and the PHUSER program.<br>
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*The construction of the four biobricks submitted will be described.<br>
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Throughout the text, links will guide to a detailed descriptions of the protocols used in each case.<br>
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<font size="3"><b>The Redoxilator</b></font><br>
 
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<p align="justify">By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast.</p>
 
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<font size="3"><b>The USER fusion standard</b></font><br>
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<p align="center"><i>Our three main tools... and Mads</i></p><br><br>
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<p align="justify">Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TradeMark) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.</p>
 
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<p align="center"><i>Hans working hard in the lab, and Bjørn working just as hard somewhere else</i><p>
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   <b>Medal quest status</b><br><br>
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   <b>Work process</b><br><br>
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We surely have the gold by now!
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<p>Our team works parallel in smaller sub-teams. Some of us work hard in the lab, while others are in the process of developing software and the <i>in silico</i> model of the Redoxilator system. However, we constantly keep each other updated, and meet often to exchange ideas and take turns at the different tasks, thus exhausting all of our combined knowledge in every aspect of this project.</p>

Latest revision as of 01:13, 22 October 2009

Wiki banner 967px.png

Welcome to the DTU iGEM wiki!

Home The Team The Project Parts submitted Modelling Notebook

Activities relating to our two sub-projects:

 - The Redoxilator
- The USER assembly standard
- Biobricks
- Protocols
Day-to-day activities


 In this section we have described our daily activities. The description of our experimental work is divided into the following sections:

  • The activities related to the design, construction and testing of the Redoxilator.
  • The events leading to the USER assembly standard and the PHUSER program.
  • The construction of the four biobricks submitted will be described.

Throughout the text, links will guide to a detailed descriptions of the protocols used in each case.
Dtuwork1.jpg

Our three main tools... and Mads



Dtuwork2.jpg

Hans working hard in the lab, and Bjørn working just as hard somewhere else

 Work process

Our team works parallel in smaller sub-teams. Some of us work hard in the lab, while others are in the process of developing software and the in silico model of the Redoxilator system. However, we constantly keep each other updated, and meet often to exchange ideas and take turns at the different tasks, thus exhausting all of our combined knowledge in every aspect of this project.
Comments or questions to the team? Please -- Comments of questions to webmaster? Please
Retrieved from "http://2009.igem.org/Team:DTU_Denmark/notebook"