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Team:UAB-Barcelona/CS1
From 2009.igem.org
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''Biobrick 10'' (X,P) + '''RBS-GFP-T-T''' BBa_I13504 (S,P) // ''Biobrick 16'' | ''Biobrick 10'' (X,P) + '''RBS-GFP-T-T''' BBa_I13504 (S,P) // ''Biobrick 16'' | ||
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| + | {|class="wikitable" border="0" cellpadding="10" cellspacing="1" style="padding: 0; background-color:black; border: 1px solid black; text-align:justify; margin:5px -5px 5px -5px" | ||
| + | !width="30%"; valign="top" style="background:white; color:black"| | ||
| + | |||
| + | =====Biobricks used===== | ||
| + | |||
| + | BBa_R0040 | ||
| + | http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 ''ptet'' P1 6I | ||
| + | |||
| + | BBa_J04450 | ||
| + | http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 | ||
| + | ''pLacI-RBS-RFP-T-T'' | ||
| + | P1 7A | ||
| + | |||
| + | BBa_J24679 | ||
| + | http://partsregistry.org/wiki/index.php?title=Part:BBa_J24679 | ||
| + | ''RBS-LacI'' | ||
| + | P1 7L | ||
| + | |||
| + | BBa_J61127 | ||
| + | http://partsregistry.org/wiki/index.php?title=Part:BBa_J61127 | ||
| + | ''RBS'' | ||
| + | P1 11N | ||
| + | |||
| + | BBa_E0840 | ||
| + | http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840 | ||
| + | ''RBS-GFP-T-T'' | ||
| + | P1 12O | ||
| + | |||
| + | BBa_P0140 | ||
| + | http://partsregistry.org/wiki/index.php?title=Part:BBa_P0140 | ||
| + | ''RBS-tetR'' | ||
| + | P2 1F | ||
| + | |||
| + | BBa_P0340 | ||
| + | http://partsregistry.org/wiki/index.php?title=Part:BBa_P0340 | ||
| + | ''RBS-tetR'' | ||
| + | P2 1J | ||
| + | |||
| + | BBa_K091112 | ||
| + | http://partsregistry.org/wiki/index.php?title=Part:BBa_K091112 | ||
| + | ''placI'' | ||
| + | P2 8B | ||
| + | |||
| + | BBa_K093005 | ||
| + | http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 | ||
| + | ''RBS-RFP-T-T'' | ||
| + | P3 13K | ||
Revision as of 01:24, 22 October 2009
Contents |
Cloning strategy
1st roundphoa (E,S) + backbone BBa_K093005 (E,S) // Biobrick 1 nirk (E,S) + backbone BBa_K093005 (E,S) // Biobrick 2 nsr (X,P) + backbone BBa_K093005 (E,S) // Biobrick 3
pLacI-RBS-RFP-T-T BBa_J04450 (E,S) + linker BBa_B0040 (E,X) // Biobrick 5 ptet-RBS-RFP-T-T BBa_I13521 (E,S) + linker BBa_B0040 (E,X) // Biobrick 6
RBS BBa_J61127 (S,P) + nsr (X,P) // Biobrick 8 ptet BBa_R0040 (S,P) + Biobrick 8 (X,P) // Biobrick 9 placI BBa_K091112 (S,P) + Biobrick 8 (X,P) // Biobrick 10
2nd roundBiobrick 4 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 11 Biobrick 7 (X,P) + RBS-GFP-T-T BBa_E0840 (S,P) // Biobrick 12
3rd roundBiobrick 11 + Biobrick 5 // Biobrick 13 Biobrick 11 + Biobrick 6 // Biobrick 14 Biobrick 9 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 15 Biobrick 10 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 16
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