Team:LCG-UNAM-Mexico:Journals:Uriel
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this is strange because the plasmid maintained its size so the promoter is not there.I've checked the plasmid size an it is 2079 bp so the plasmid doesn't has the promoter. | this is strange because the plasmid maintained its size so the promoter is not there.I've checked the plasmid size an it is 2079 bp so the plasmid doesn't has the promoter. | ||
- | Going back further when I digested for the first time plasmid | + | Going back further when I digested for the first time plasmid [http://partsregistry.org/Part:pSB1A2 pSB1A2] I did it with SpeI/PstI to allow |
promoter to be at the beginning of the construction.The insert [http://partsregistry.org/Part:BBa_K242002 cox]+[http://partsregistry.org/Part:BBa_K242001 ogr]+[http://partsregistry.org/Part:BBa_B0015 BBa_B0015] was digested XbaI/PstI so I think the unique alternative is that promoter ipteg wasn't | promoter to be at the beginning of the construction.The insert [http://partsregistry.org/Part:BBa_K242002 cox]+[http://partsregistry.org/Part:BBa_K242001 ogr]+[http://partsregistry.org/Part:BBa_B0015 BBa_B0015] was digested XbaI/PstI so I think the unique alternative is that promoter ipteg wasn't | ||
in the plasmid. | in the plasmid. | ||
- | In this circumstance we can only say that we changed [http://partsregistry.org/Part:BBa_K242002 cox]+[http://partsregistry.org/Part:BBa_K242001 ogr]+[http://partsregistry.org/Part:BBa_B0015 BBa_B0015] from plasmid | + | In this circumstance we can only say that we changed [http://partsregistry.org/Part:BBa_K242002 cox]+[http://partsregistry.org/Part:BBa_K242001 ogr]+[http://partsregistry.org/Part:BBa_B0015 BBa_B0015] from plasmid [http://partsregistry.org/Part:pSB1T3 pSB1T3] to [http://partsregistry.org/Part:pSB1A212 pSB1A212] that is resitantant to |
Amp. | Amp. | ||
Revision as of 01:28, 22 October 2009