Team:Utah State/Project

From 2009.igem.org

(Difference between revisions)
 
(24 intermediate revisions not shown)
Line 10: Line 10:
<head>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
<title>Untitled Document</title>
+
<p class="class"><title>Untitled Document</title>
</head>
</head>
<style type="text/css">
<style type="text/css">
Line 17: Line 17:
display : block;
display : block;
color : #999;
color : #999;
-
font-weight : none;  
+
p class="class"> font-weight : none;  
font-family:Century Gothic, Arial, sans-serif;
font-family:Century Gothic, Arial, sans-serif;
text-decoration : none;
text-decoration : none;
Line 82: Line 82:
       </tr>
       </tr>
       <tr>
       <tr>
-
         <td width="172" id="ana"><span class="currentPage"><font size = 4>PROJECT</font></span><a href="#abstract">Abstract</a><br />
+
         <td width="172" id="ana"><span class="currentPage"><font size = 4>PROJECT</font></span>
-
        <a href="#introduction">Introduction</a><br />
+
 
-
         <a href="#broadhostvector">Broad-Host Vectors</a><br />
+
        <a href="https://2009.igem.org/Team:Utah_State/Abstract">Abstract</a><br />
-
         <a href="#secretion">Secretion</a>
+
<a href="https://2009.igem.org/Team:Utah_State/Introduction">Introduction</a><br />
-
        <a href="#experiments">Experiments</a><br />
+
         <a href="https://2009.igem.org/Team:Utah_State/Broad-HostVectors">Broad-Host Vectors</a><br />
-
        <a href="#conclusions">Conclusions</a>
+
         <a href="https://2009.igem.org/Team:Utah_State/Secretion">Secretion</a><br />
-
         <a href="#references">References</a>
+
        <a href="https://2009.igem.org/Team:Utah_State/Experiments">Experiments</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/FutureWork">Future Work</a><br />
 +
         <a href="https://2009.igem.org/Team:Utah_State/References">References</a><br />
       </tr>
       </tr>
-
      <tr>
+
      <tr>
         <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Parts"><font size = 4>BIOBRICKS</font></a></td>
         <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Parts"><font size = 4>BIOBRICKS</font></a></td>
       </tr>
       </tr>
-
      <tr>
+
        <tr>
       <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Modeling"><font size = 4>MODELING</font></a></td>
       <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Modeling"><font size = 4>MODELING</font></a></td>
       </tr>
       </tr>
Line 100: Line 102:
       </tr>
       </tr>
       <tr>
       <tr>
-
       <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Notebook"><font size = 4>ETHICS</font></a></td>
+
       <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/ETHICS"><font size = 4>ETHICS</font></a></td>
       </tr>
       </tr>
       <tr>
       <tr>
-
        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Links"><font size = 4>SUCCESS</font></a></td>
+
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Achievements"><font size = 4>JUDGING</font></a></td>
 +
      </tr>
 +
    <tr>
 +
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Contact"><font size = 4>CONTACT</font></a></td>
       </tr>
       </tr>
     </table></td>
     </table></td>
-
   
 
<!---TEXT BOXES--->
<!---TEXT BOXES--->
     <td><table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
     <td><table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
   </td></tr>
   </td></tr>
-
<tr>
+
 
-
    <td>
+
-
      <a name="abstract">
+
-
    </td>
+
-
    </tr>
+
     <tr>
     <tr>
     <td>
     <td>
Line 131: Line 131:
   </P> </td></tr>
   </P> </td></tr>
-
<!---Go To Top of Page--->
+
    
-
 
+
-
  <tr>
+
-
    <td>
+
-
      <font size="3" face="Century Gothic, Arial, San Serif" color =#000033 <div align="center">
+
-
   <b><a href="#top">Go to Top of Page</a></b>
+
-
</div> </font>
+
-
    </td>
+
-
    </tr>
+
-
 
+
-
</table>
+
-
 
+
-
<!---Introduction--->
+
-
 
+
-
<br>
+
-
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
+
-
  <tr>
+
-
    <td>
+
-
</td></tr>
+
-
<tr>
+
-
    <td>
+
-
      <a name="introduction">
+
-
    </td>
+
-
    </tr>
+
-
    <tr>
+
-
    <td>
+
-
    <b><i><font size="3" face="Helvetica, Arial, San Serif" color =#000033>
+
-
Introduction
+
-
    </font></b></i> <hr>
+
-
<p class = "class">
+
-
Introductory Text
+
-
</p>
+
   </td>
   </td>
   </tr></tr>
   </tr></tr>
   </table>
   </table>
-
<br>
 
-
 
-
<!---Broad-Host Range Vectors--->
 
-
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 
-
  <tr>
 
-
    <td>
 
-
</td></tr>
 
-
<tr>
 
-
    <td>
 
-
      <a name="broadhostvectors">
 
-
    </td>
 
-
    </tr>
 
-
    <tr>
 
-
    <td>
 
-
    <b><i><font size="3" face="Helvetica, Arial, San Serif" color =#000033>
 
-
Broad-Host Range Vectors
 
-
    </font></b></i> <hr>
 
-
   
 
-
<p class="class">
 
-
Vector text
 
-
</p>
 
-
 
-
    </font></p>
 
-
  </td>
 
-
  </tr></tr>
 
-
 
-
<!---Go To Top of Page--->
 
-
 
-
  <tr>
 
-
    <td>
 
-
      <font size="3" face="Century Gothic, Arial, San Serif" color =#000033 <div align="center">
 
-
  <b><a href="#top">Go to Top of Page</a></b>
 
-
</div> </font>
 
-
    </td>
 
-
    </tr>
 
-
 
-
  </table>
 
-
 
-
<!---Secretion Mechanisms--->
 
-
<br>
 
-
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 
-
  <tr>
 
-
    <td>
 
-
</td></tr>
 
-
<tr>
 
-
    <td>
 
-
      <a name="secretion">
 
-
    </td>
 
-
    </tr>
 
-
    <tr>
 
-
    <td>
 
-
    <b><i><font size="3" face="Helvetica, Arial, San Serif" color =#000033>
 
-
Secretion: GFP, Phasins, and Bioplastics
 
-
    </font></b></i> <hr>
 
-
<p class="class">
 
-
This is where all of the text about secretion goes
 
-
</p>
 
-
 
-
  </td>
 
-
  </tr></tr>
 
-
<!---Go To Top of Page--->
 
-
 
-
  <tr>
 
-
    <td>
 
-
      <font size="3" face="Century Gothic, Arial, San Serif" color =#000033 <div align="center">
 
-
  <b><a href="#top">Go to Top of Page</a></b>
 
-
</div> </font>
 
-
    </td>
 
-
    </tr>
 
-
 
-
  </table>
 
-
 
-
<!---Experiments--->
 
-
<br>
 
-
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 
-
  <tr>
 
-
    <td>
 
-
</td></tr>
 
-
<tr>
 
-
    <td>
 
-
      <a name="experiments">
 
-
    </td>
 
-
    </tr>
 
-
    <tr>
 
-
    <td>
 
-
    <b><i><font size="3" face="Helvetica, Arial, San Serif" color =#000033>
 
-
Experiments
 
-
    </font></b></i> <hr>
 
-
<p class="class">
 
-
This is where all experimental text goes!
 
-
</p>
 
-
    </p>
 
-
  </td>
 
-
  </tr></tr>
 
-
<!---Go To Top of Page--->
 
-
 
-
  <tr>
 
-
    <td>
 
-
      <font size="3" face="Century Gothic, Arial, San Serif" color =#000033 <div align="center">
 
-
  <b><a href="#top">Go to Top of Page</a></b>
 
-
</div> </font>
 
-
    </td>
 
-
    </tr>
 
-
  </table>
 
-
 
-
<!---Conclusions--->
 
-
<br>
 
-
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 
-
  <tr>
 
-
    <td>
 
-
</td></tr>
 
-
<tr>
 
-
    <td>
 
-
      <a name="conclusions">
 
-
    </td>
 
-
    </tr>
 
-
    <tr>
 
-
    <td>
 
-
    <b><i><font size="3" face="Helvetica, Arial, San Serif" color =#000033>
 
-
Conclusions
 
-
    </font></b></i> <hr>
 
-
<p class="class">
 
-
Conclusions go here
 
-
 
-
</p>
 
-
  </td>
 
-
<!---Go To Top of Page--->
 
-
 
-
  <tr>
 
-
    <td>
 
-
      <font size="3" face="Century Gothic, Arial, San Serif" color =#000033 <div align="center">
 
-
  <b><a href="#top">Go to Top of Page</a></b>
 
-
</div> </font>
 
-
    </td>
 
-
    </tr>
 
-
  </tr></tr>
 
-
  </table>
 
-
 
-
<!---References--->
 
-
<br>
 
-
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 
-
  <tr>
 
-
    <td>
 
-
</td></tr>
 
-
<tr>
 
-
    <td>
 
-
      <a name="references">
 
-
    </td>
 
-
    </tr>
 
-
    <tr>
 
-
    <td>
 
-
    <b><i><font size="3" face="Helvetica, Arial, San Serif" color =#000033>
 
-
References
 
-
    </font></b></i> <hr>
 
-
<ul class=circle>
 
-
<li>This is where references go</li>
 
-
</ul>
 
-
 
-
   
 
-
  </td>
 
-
  </tr></tr>
 
-
  </table>
 
-
 
-
 
</td>
</td>
   </tr>
   </tr>

Latest revision as of 02:00, 22 October 2009

USU iGem

Untitled Document

PROJECT Abstract
Introduction
Broad-Host Vectors
Secretion
Experiments
Future Work
References
BioBricks without Borders:
Investigating a multi-host BioBrick vector and secretion of cellular products

The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.



Retrieved from "http://2009.igem.org/Team:Utah_State/Project"