Team:Utah State/Project

From 2009.igem.org

(Difference between revisions)
 
(44 intermediate revisions not shown)
Line 1: Line 1:
 +
<!--- BANNER--->
<html>
<html>
<a href="https://2009.igem.org/Team:Utah_State">
<a href="https://2009.igem.org/Team:Utah_State">
<img alt="USU iGem" src="https://static.igem.org/mediawiki/2009/7/71/USUlogo.jpg"/> </a>
<img alt="USU iGem" src="https://static.igem.org/mediawiki/2009/7/71/USUlogo.jpg"/> </a>
-
 
-
 
-
 
Line 12: Line 10:
<head>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
<title>Untitled Document</title>
+
<p class="class"><title>Untitled Document</title>
</head>
</head>
<style type="text/css">
<style type="text/css">
#ana {
#ana {
-
width: 6em;
+
width: 11em;
display : block;
display : block;
color : #999;
color : #999;
-
font-weight : none;  
+
p class="class"> font-weight : none;  
font-family:Century Gothic, Arial, sans-serif;
font-family:Century Gothic, Arial, sans-serif;
text-decoration : none;
text-decoration : none;
Line 27: Line 25:
#ana .currentPage {
#ana .currentPage {
-
padding-left:15px;
+
padding-left:9px;
         border-top-style: none;
         border-top-style: none;
color : #56b8df;
color : #56b8df;
Line 41: Line 39:
font-size:x-small;
font-size:x-small;
text-decoration : none;
text-decoration : none;
-
padding-left:45px;
+
padding-left:17px;
color : #E0FFFF;
color : #E0FFFF;
font-family: Verdana, Arial, sans-serif;
font-family: Verdana, Arial, sans-serif;
Line 54: Line 52:
#nav a {
#nav a {
-
padding-left: 15px;
+
padding-left: 9px;
width:10em;
width:10em;
display : block;
display : block;
Line 78: Line 76:
     <td width="16%" valign="top"><table width="72%" border="0">
     <td width="16%" valign="top"><table width="72%" border="0">
       <tr valign="top">
       <tr valign="top">
-
         <td width="172" id="ana"><span class="currentPage"><font size = 4>HOME</font></span>
+
         <td id="nav"><a href="https://2009.igem.org/Team:Utah_State"><font size = 4>HOME</font></a></td>
       </tr>
       </tr>
       <tr>
       <tr>
Line 84: Line 82:
       </tr>
       </tr>
       <tr>
       <tr>
-
         <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Project"><font size = 4>PROJECT</font></a></td>
+
         <td width="172" id="ana"><span class="currentPage"><font size = 4>PROJECT</font></span>
 +
 
 +
        <a href="https://2009.igem.org/Team:Utah_State/Abstract">Abstract</a><br />
 +
<a href="https://2009.igem.org/Team:Utah_State/Introduction">Introduction</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/Broad-HostVectors">Broad-Host Vectors</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/Secretion">Secretion</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/Experiments">Experiments</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/FutureWork">Future Work</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/References">References</a><br />
       </tr>
       </tr>
-
      <tr>
+
      <tr>
         <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Parts"><font size = 4>BIOBRICKS</font></a></td>
         <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Parts"><font size = 4>BIOBRICKS</font></a></td>
 +
      </tr>
 +
        <tr>
 +
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Modeling"><font size = 4>MODELING</font></a></td>
       </tr>
       </tr>
       <tr>
       <tr>
       <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Notebook"><font size = 4>NOTEBOOK</font></a></td>
       <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Notebook"><font size = 4>NOTEBOOK</font></a></td>
 +
      </tr>
 +
      <tr>
 +
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/ETHICS"><font size = 4>ETHICS</font></a></td>
       </tr>
       </tr>
       <tr>
       <tr>
-
        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Links"><font size = 3>SUCCESS</font></a></td>
+
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Achievements"><font size = 4>JUDGING</font></a></td>
 +
      </tr>
 +
    <tr>
 +
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Contact"><font size = 4>CONTACT</font></a></td>
       </tr>
       </tr>
     </table></td>
     </table></td>
 +
<!---TEXT BOXES--->
     <td><table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
     <td><table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 +
  </td></tr>
 +
 +
    <tr>
 +
    <td>
   <tr>
   <tr>
     <td>
     <td>
-
       <font size="6" face="Century Gothic, Arial, San Serif" color =#000033>
+
       <font size="5" face="Century Gothic, Arial, San Serif" color =#000033>
-
               <b><i>BioBricks without Borders:</b></i></font>
+
               <b><i>BioBricks without Borders:
-
              <p><font face="Helvetica, Arial, San Serif" color =green>Investigating a multi-host BioBrick vector and secretion of cellular products</font></p><HR>
+
</b></i></font>
-
<p> <font size="2.5" face=" Tahoma, Helvetica, Arial" color =#000000>The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like <i>Pseudomonas putida</i>, <i>Rhodobacter sphaeroides</i>, and <i>Synechocystis</i> PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.</p>
+
<br>
-
          </font>
+
<font size ="2.5" face="tahoma, sans-serif, verdana" color=#009900>
-
            <p> <font size="4" face="Helvetica, Arial, San Serif" color =green>OUR SITE IS STILL UNDER CONSTRUCTION AND OUR INFORMATION IS BEING ADDED.  PLEASE COME BACK IN A FEW WEEKS TO SEE OUR PROJECT!</font></p>
+
  Investigating a multi-host BioBrick vector and secretion of cellular products</font>
-
    </td>
+
              <HR>
-
    </tr>
+
 
-
  </tr>
+
  <p class = "class">  
 +
The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.
 +
  </P> </td></tr>
 +
 
 +
 
 +
  </td>
 +
  </tr></tr>
 +
  </table>
 +
</td>
 +
  </tr>
</table>
</table>
 +
</body>
</html>
</html>
 +
<!---BACKGROUND FORMATTING--->
<html>
<html>
<head>
<head>
Line 155: Line 186:
</html>
</html>
-
<html>
 
-
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 
-
 
-
  <tr>
 
-
    <td>
 
-
  This is where more text can go
 
-
   
 
-
   
 
-
    </td>
 
-
   
 
-
  </tr>
 
-
 
-
  </tr>
 
-
</table></td>
 
-
  </tr>
 
-
</table>
 
-
</body>
 
-
</html>
 
Line 187: Line 200:
</html>
</html>
-
 
+
<html>
-
 
+
<head>
-
<!--- Ty's Flash Section--->
+
<style type="text/css">
-
<html><p>
+
p.class{
-
<object classid="clsid:D27CDB6E-AE6D-11CF-96B8-444553540000" id="obj1" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,40,0" border="0" width="965" height="768">
+
text-indent:1.0em;
-
<param name="movie" value="https://static.igem.org/mediawiki/2009/e/ed/Firsteditionusuflash.swf">
+
color: #231f20;
-
<param name="quality" value="High">
+
font-weight : none;
-
<embed src="https://static.igem.org/mediawiki/2009/e/ed/Firsteditionusuflash.swf" pluginspage="http://www.macromedia.com
+
font-family:Tahoma, Arial, Sans-serif;
-
/go/getflashplayer" type="application/x-shockwave-flash" name="obj1" width="400" height="300"  quality="High"></object>
+
font size="2.5";
-
</p>
+
}
-
</html>
+
p.margin
 +
{
 +
margin-top:0px;
 +
margin-bottom:0px;
 +
margin-right:50px;
 +
margin-left:50px;
 +
color: #231f20;
 +
font-weight : none;
 +
font-family:Tahoma, Arial, Sans-serif;
 +
font size="2.5";
 +
font-style: italic;
 +
}
 +
ul.circle {list-style-type:circle; color: #231f20;}
 +
</style>
 +
</head>

Latest revision as of 02:00, 22 October 2009

USU iGem

Untitled Document

PROJECT Abstract
Introduction
Broad-Host Vectors
Secretion
Experiments
Future Work
References
BioBricks without Borders:
Investigating a multi-host BioBrick vector and secretion of cellular products

The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.



Retrieved from "http://2009.igem.org/Team:Utah_State/Project"