Team:DTU Denmark/notebookredoxilator

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     <a href="https://2009.igem.org/Team:DTU_Denmark/notebookredoxilator" CLASS=leftbar>- The Redoxilator</a><br>
     <a href="https://2009.igem.org/Team:DTU_Denmark/notebookredoxilator" CLASS=leftbar>- The Redoxilator</a><br>
     <a href="https://2009.igem.org/Team:DTU_Denmark/notebookuserfusion" CLASS=leftbar>- The USER<sup>TM</sup> assembly standard</a><br>
     <a href="https://2009.igem.org/Team:DTU_Denmark/notebookuserfusion" CLASS=leftbar>- The USER<sup>TM</sup> assembly standard</a><br>
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    <a href="https://2009.igem.org/Team:DTU_Denmark/notebookbiobrick" CLASS=leftbar>- Biobricks</a><br>
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    <a href="https://2009.igem.org/Team:DTU_Denmark/protocols" CLASS=leftbar>- Protocols</a><br>
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<font size="4"><b>Activities relating to the redoxilator</b></font><br>
<font size="4"><b>Activities relating to the redoxilator</b></font><br>
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For more detailed information see <a href="https://2009.igem.org/Team:DTU_Denmark/results"> results</a>. <br><br>
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<font size="3"><b> Thursday 15/10/2009 </b></font><br>
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BioLector experiment initiated! Lets get some results!
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<br><br>
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<font size="3"><b> Wednesday 14/10/2009 </b></font><br>
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To have inoculum for the BioLector experiment, colonies were picked from our plates and grown till tomorrow.
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<br><br>
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<font size="3"><b>July 20th</b></font><br>
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<font size="3"><b> Monday 5/10/2009 </b></font><br>
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<p align="justify">Construction of biobricks: The restriction products were evaluated on a 1% agarose gel (see figure 2).</p><br>
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The yeasts were streak diluted onto a new plate to be sure that we have a single transformant.
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<br><br>
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<font size="3"><b> Thursday 1/10/2009 </b></font><br>
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[[image:DTUgel1.jpg]]
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Our redoxilator construct and <i>leaky</i> construct were ready for yeast transformation into CEN PK 113 5D (Ura-) using the URA marker present these plasmids.<br><br>
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<p align="center"><i>Figure 2: Restriction analysis of the three biobricks.</i>
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<font size="3"><b>July 19th</b></font><br>
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<font size="3"><b> Wednesday 30/9/2009 </b></font><br>
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<p align="justify">Construction of biobricks: The three plasmids were purified according to the protocol (link to protocol). Following the purification of the plasmids, a restriction analysis was performed using SpeI enzyme (10U) and incubated at 37ºC overnight.</p><br>
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The resulting vectors were purification. The presence of our leaky redoxilator construct was verified by digestion with XmaI.<br><br>
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<font size="3"><b>July 18th</b></font><br>
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<font size="3"><b> Tuesday 29/9/2009 </b></font><br>
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<p align="justify">Construction of biobricks: Some colonies were picked from the transformation plates and made overnight cultures in liquid media.</p><br>
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Liquid over night colonies were prepared. Cultures were randomly picked using a sterile pipette tip from the transformation plate and incubated into liquid media containing kanamycin. <br><br>
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<font size="3"><b>July 17th</b></font><br>
 
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<p align="justify">Construction of biobricks:        </p><br>
 
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<font size="3"><b>July 16th</b></font><br>
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<font size="3"><b> Monday 28/9/2009 </b></font><br>
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<p align="justify">Construction of biobricks:        </p><br>
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To test how leaky our redoxilator construct was, the redoxilator part was removed by NotI digested followed by gel-purification. The backbone missing the redox sensor/activator was ligated and transformed into <i>E. coli</i>.<br><br>
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<font size="3"><b>July 15th</b></font><br>
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<font size="3"><b> Friday 25/9/2009 </b></font><br>
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<p align="justify">Construction of biobricks:        </p><br>
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Colonies seen!<br><br>
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<font size="3"><b>July 14th</b></font><br>
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<font size="3"><b> Thursday 24/9/2009 </b></font><br>
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<p align="justify">Construction of biobricks:        </p><br>
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Our dear redoxilator construct arrieved from California (DNA 2.0)
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The DNA was extracted from filter paper using MilliQ water and transformed into <i>E. Coli</i>.<br><br>
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<font size="3"><b>July 13th</b></font><br>
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<font size="3"><b> June 2009 </b></font><br>
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<p align="justify">Construction of biobricks:        </p><br>
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The genetic design is now ready, and it should work according to the newly finished model. <br><br>
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<font size="3"><b> Since January 2009... </b></font><br>
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Figuring out the project idea took a while and meetings among the team members started early this year.

Latest revision as of 02:31, 22 October 2009

Wiki banner 967px.png

Welcome to the DTU iGEM wiki!

Home The Team The Project Parts submitted Modelling Notebook

Activities relating to our two sub-projects:

 - The Redoxilator
- The USERTM assembly standard
- Biobricks
- Protocols
Day-to-day activities


 Activities relating to the redoxilator
For more detailed information see results.

Thursday 15/10/2009
BioLector experiment initiated! Lets get some results!

Wednesday 14/10/2009
To have inoculum for the BioLector experiment, colonies were picked from our plates and grown till tomorrow.

Monday 5/10/2009
The yeasts were streak diluted onto a new plate to be sure that we have a single transformant.

Thursday 1/10/2009
Our redoxilator construct and leaky construct were ready for yeast transformation into CEN PK 113 5D (Ura-) using the URA marker present these plasmids.

Wednesday 30/9/2009
The resulting vectors were purification. The presence of our leaky redoxilator construct was verified by digestion with XmaI.

Tuesday 29/9/2009
Liquid over night colonies were prepared. Cultures were randomly picked using a sterile pipette tip from the transformation plate and incubated into liquid media containing kanamycin.

Monday 28/9/2009
To test how leaky our redoxilator construct was, the redoxilator part was removed by NotI digested followed by gel-purification. The backbone missing the redox sensor/activator was ligated and transformed into E. coli.

Friday 25/9/2009
Colonies seen!

Thursday 24/9/2009
Our dear redoxilator construct arrieved from California (DNA 2.0) The DNA was extracted from filter paper using MilliQ water and transformed into E. Coli.

June 2009
The genetic design is now ready, and it should work according to the newly finished model.

Since January 2009...
Figuring out the project idea took a while and meetings among the team members started early this year. 		Work process

Our team works parallel in smaller sub-teams. Some of us work hard in the lab, while others are in the process of developing software and the in silico model of the Redoxilator system. However, we constantly keep each other updated, and meet often to exchange ideas and take turns at the different tasks, thus exhausting all of our combined knowledge in every aspect of this project.
Comments or questions to the team? Please -- Comments of questions to webmaster? Please

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