Team:UC Davis/wiring pH sensor

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<br>
<br>
Th pH sensor: </span></big><br>
Th pH sensor: </span></big><br>
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&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; ChvG is a
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&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; ChvG/ChvI is a
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suspected pH sensor and histidine kinase and ChvI is its
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two-component system and a suspected pH sensor. ChvG is the histidine
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cognate response regulator (2). Together they are believed to sense
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kinase and ChvI is its
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extracellular pH between 5.5 to 7 and relaying this signal to regulate
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cognate response regulator(2). Together they are believed to sense
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the expression of key virulence related genes. (2)<br>
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extracellular pH between 5.5 to 7 and relay this signal to regulate
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the expression of key virulence-related genes(2).<br>
In our design, we used this two-component system from <i>Agrobacterium
In our design, we used this two-component system from <i>Agrobacterium
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tumefaciens</i>, to regulate the expression of select target genes.<br>
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tumefaciens</i> to regulate the expression of select target genes.<br>
<hr style="width: 100%; height: 2px;"><big><span
<hr style="width: 100%; height: 2px;"><big><span
style="text-decoration: underline; font-weight: bold;">Possibility of
style="text-decoration: underline; font-weight: bold;">Possibility of
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Cross Talk:</span></big><br>
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Cross-Talk:</span></big><br>
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; Bacteria have
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; Bacteria have
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numerous related kinase/response regulated based, two component systems
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numerous related two-component systems based on
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(5). For instance, the <i>Agrobacterium tumefaciens</i>
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kinase/response-regulation(5). For instance, the <i>A. tumefaciens</i>
genome is known to contain at least 25 two-component pathways
genome is known to contain at least 25 two-component pathways
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(2).Therefore, there are possibilities of crosstalk between some genes
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(2). Therefore, there are possibilities of cross-talk between some
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present in <i>E. coli</i> that have the same mechanism as ChvI/ChvG
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genes
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present in <i>E. coli</i> that have the same mechanism as the
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ChvI/ChvG
system. In fact, there is evidence supporting that ChvI can complement
system. In fact, there is evidence supporting that ChvI can complement
a PhoB deletion in <i>E. coli</i>. (3) Therefore it is highly likely
a PhoB deletion in <i>E. coli</i>. (3) Therefore it is highly likely
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that there is a possibility of cross talk between our new sensory
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that there is a possibility of cross-talk between our new sensory
systems elements, present in <i>E. coli</i>.
systems elements, present in <i>E. coli</i>.
Many protein-protein interactions are not fully understood (5), which
Many protein-protein interactions are not fully understood (5), which
makes this project more challenging but very interesting.
makes this project more challenging but very interesting.
<br>
<br>
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<br>
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<big><span style="font-weight: bold; text-decoration: underline;"> </span></big>
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src="https://static.igem.org/mediawiki/2009/e/ea/UCDAVIS_pH_sensor1.jpg"><br>
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<span style="font-style: italic;"></span></div>
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<span style="font-style: italic;">Click on the specific part or
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interaction for more information.<br>
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Parts: <a href="https://2009.igem.org/Team:UC_Davis/Project1/ChvG.html">ChvG</a>,<a
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href="https://2009.igem.org/Team:UC_Davis/ChvI1"> ChvI</a>,<a
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href="https://2009.igem.org/Team:UC_Davis/PhoR1"> PhoR</a>,<a
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href="https://2009.igem.org/Team:UC_Davis/PhoB1"> PhoB</a><br>
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Interactions: <a href="https://2009.igem.org/Team:UC_Davis/ChvG_ChvI">ChvG---&gt;
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ChvI</a> , <a href="https://2009.igem.org/Team:UC_Davis/PhoR_ChvI1">PhoR---&gt;
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ChvI </a>, <a href="https://2009.igem.org/Team:UC_Davis/PhoR_PhoB">PhoR---&gt;PhoB</a>
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,<a href="https://2009.igem.org/Team:UC_Davis/ChvI_PhoA">ChvI---&gt;PhoA<br>
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</a><br>
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</span><big><span style="font-weight: bold; text-decoration: underline;">
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</span></big>
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Latest revision as of 02:41, 22 October 2009

 
Wiring the pH sensor into our system:

Th pH sensor:
            ChvG/ChvI is a two-component system and a suspected pH sensor. ChvG is the histidine kinase and ChvI is its cognate response regulator(2). Together they are believed to sense extracellular pH between 5.5 to 7 and relay this signal to regulate the expression of key virulence-related genes(2).
In our design, we used this two-component system from Agrobacterium tumefaciens to regulate the expression of select target genes.
Possibility of Cross-Talk:
            Bacteria have numerous related two-component systems based on kinase/response-regulation(5). For instance, the A. tumefaciens genome is known to contain at least 25 two-component pathways (2). Therefore, there are possibilities of cross-talk between some genes present in E. coli that have the same mechanism as the ChvI/ChvG system. In fact, there is evidence supporting that ChvI can complement a PhoB deletion in E. coli. (3) Therefore it is highly likely that there is a possibility of cross-talk between our new sensory systems elements, present in E. coli. Many protein-protein interactions are not fully understood (5), which makes this project more challenging but very interesting.

Click on the specific part or interaction for more information.
Parts: ChvG, ChvI, PhoR, PhoB
Interactions: ChvG---> ChvI , PhoR---> ChvI , PhoR--->PhoB ,ChvI--->PhoA

Retrieved from "http://2009.igem.org/Team:UC_Davis/wiring_pH_sensor"