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Team:UC Davis/wiring pH sensor
From 2009.igem.org
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style="border: 0px solid ; width: 83px; height: 37px;"></a></big></big></big></big></span></b><b | style="border: 0px solid ; width: 83px; height: 37px;"></a></big></big></big></big></span></b><b | ||
style="color: rgb(0, 0, 0);"><span | style="color: rgb(0, 0, 0);"><span | ||
| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";" | + | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"></span><br> |
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<hr style="width: 100%; height: 2px;"> | <hr style="width: 100%; height: 2px;"> | ||
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Th pH sensor: </span></big><br> | Th pH sensor: </span></big><br> | ||
| - | ChvG is a | + | ChvG/ChvI is a |
| - | suspected pH sensor | + | two-component system and a suspected pH sensor. ChvG is the histidine |
| - | cognate response regulator (2). Together they are believed to sense | + | kinase and ChvI is its |
| - | extracellular pH between 5.5 to 7 and | + | cognate response regulator(2). Together they are believed to sense |
| - | the expression of key virulence related genes | + | extracellular pH between 5.5 to 7 and relay this signal to regulate |
| + | the expression of key virulence-related genes(2).<br> | ||
In our design, we used this two-component system from <i>Agrobacterium | In our design, we used this two-component system from <i>Agrobacterium | ||
| - | tumefaciens</i> | + | tumefaciens</i> to regulate the expression of select target genes.<br> |
<hr style="width: 100%; height: 2px;"><big><span | <hr style="width: 100%; height: 2px;"><big><span | ||
style="text-decoration: underline; font-weight: bold;">Possibility of | style="text-decoration: underline; font-weight: bold;">Possibility of | ||
| - | Cross Talk:</span></big><br> | + | Cross-Talk:</span></big><br> |
Bacteria have | Bacteria have | ||
| - | numerous related | + | numerous related two-component systems based on |
| - | (5). For instance, the <i> | + | kinase/response-regulation(5). For instance, the <i>A. tumefaciens</i> |
genome is known to contain at least 25 two-component pathways | genome is known to contain at least 25 two-component pathways | ||
| - | (2).Therefore, there are possibilities of | + | (2). Therefore, there are possibilities of cross-talk between some |
| - | present in <i>E. coli</i> that have the same mechanism as ChvI/ChvG | + | genes |
| + | present in <i>E. coli</i> that have the same mechanism as the | ||
| + | ChvI/ChvG | ||
system. In fact, there is evidence supporting that ChvI can complement | system. In fact, there is evidence supporting that ChvI can complement | ||
a PhoB deletion in <i>E. coli</i>. (3) Therefore it is highly likely | a PhoB deletion in <i>E. coli</i>. (3) Therefore it is highly likely | ||
| - | that there is a possibility of cross talk between our new sensory | + | that there is a possibility of cross-talk between our new sensory |
systems elements, present in <i>E. coli</i>. | systems elements, present in <i>E. coli</i>. | ||
Many protein-protein interactions are not fully understood (5), which | Many protein-protein interactions are not fully understood (5), which | ||
makes this project more challenging but very interesting. | makes this project more challenging but very interesting. | ||
<br> | <br> | ||
| - | <hr style="width: 100%; height: 2px;"><br> | + | <hr style="width: 100%; height: 2px;"> |
| - | <br> | + | <div style="text-align: center;"><span style="font-style: italic;"></span><img |
| - | <big><span style="font-weight: bold; text-decoration: underline;"> </span></big> | + | style="width: 809px; height: 464px;" alt="" |
| + | src="https://static.igem.org/mediawiki/2009/e/ea/UCDAVIS_pH_sensor1.jpg"><br> | ||
| + | <span style="font-style: italic;"></span></div> | ||
| + | <span style="font-style: italic;">Click on the specific part or | ||
| + | interaction for more information.<br> | ||
| + | Parts: <a href="https://2009.igem.org/Team:UC_Davis/Project1/ChvG.html">ChvG</a>,<a | ||
| + | href="https://2009.igem.org/Team:UC_Davis/ChvI1"> ChvI</a>,<a | ||
| + | href="https://2009.igem.org/Team:UC_Davis/PhoR1"> PhoR</a>,<a | ||
| + | href="https://2009.igem.org/Team:UC_Davis/PhoB1"> PhoB</a><br> | ||
| + | Interactions: <a href="https://2009.igem.org/Team:UC_Davis/ChvG_ChvI">ChvG---> | ||
| + | ChvI</a> , <a href="https://2009.igem.org/Team:UC_Davis/PhoR_ChvI1">PhoR---> | ||
| + | ChvI </a>, <a href="https://2009.igem.org/Team:UC_Davis/PhoR_PhoB">PhoR--->PhoB</a> | ||
| + | ,<a href="https://2009.igem.org/Team:UC_Davis/ChvI_PhoA">ChvI--->PhoA<br> | ||
| + | </a><br> | ||
| + | </span><big><span style="font-weight: bold; text-decoration: underline;"> | ||
| + | </span></big> | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
| - | style="background: white none repeat scroll 0%; text-indent: 0.5in; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><b><span | + | style="background: white none repeat scroll 0% 50%; text-indent: 0.5in; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><b><span |
style="font-size: 14pt; font-family: "Times New Roman","serif"; color: black;"><o:p></o:p></span></b></p> | style="font-size: 14pt; font-family: "Times New Roman","serif"; color: black;"><o:p></o:p></span></b></p> | ||
</div> | </div> | ||
Latest revision as of 02:41, 22 October 2009
Wiring the pH
sensor into our system:
Th pH sensor:
ChvG/ChvI is a two-component system and a suspected pH sensor. ChvG is the histidine kinase and ChvI is its cognate response regulator(2). Together they are believed to sense extracellular pH between 5.5 to 7 and relay this signal to regulate the expression of key virulence-related genes(2).
In our design, we used this two-component system from Agrobacterium tumefaciens to regulate the expression of select target genes.
Possibility of Cross-Talk:
Bacteria have numerous related two-component systems based on kinase/response-regulation(5). For instance, the A. tumefaciens genome is known to contain at least 25 two-component pathways (2). Therefore, there are possibilities of cross-talk between some genes present in E. coli that have the same mechanism as the ChvI/ChvG system. In fact, there is evidence supporting that ChvI can complement a PhoB deletion in E. coli. (3) Therefore it is highly likely that there is a possibility of cross-talk between our new sensory systems elements, present in E. coli. Many protein-protein interactions are not fully understood (5), which makes this project more challenging but very interesting.
Click on the specific part or
interaction for more information.
Parts: ChvG, ChvI, PhoR, PhoB
Interactions: ChvG---> ChvI , PhoR---> ChvI , PhoR--->PhoB ,ChvI--->PhoA
Th pH sensor:
ChvG/ChvI is a two-component system and a suspected pH sensor. ChvG is the histidine kinase and ChvI is its cognate response regulator(2). Together they are believed to sense extracellular pH between 5.5 to 7 and relay this signal to regulate the expression of key virulence-related genes(2).
In our design, we used this two-component system from Agrobacterium tumefaciens to regulate the expression of select target genes.
Possibility of Cross-Talk:
Bacteria have numerous related two-component systems based on kinase/response-regulation(5). For instance, the A. tumefaciens genome is known to contain at least 25 two-component pathways (2). Therefore, there are possibilities of cross-talk between some genes present in E. coli that have the same mechanism as the ChvI/ChvG system. In fact, there is evidence supporting that ChvI can complement a PhoB deletion in E. coli. (3) Therefore it is highly likely that there is a possibility of cross-talk between our new sensory systems elements, present in E. coli. Many protein-protein interactions are not fully understood (5), which makes this project more challenging but very interesting.
Parts: ChvG, ChvI, PhoR, PhoB
Interactions: ChvG---> ChvI , PhoR---> ChvI , PhoR--->PhoB ,ChvI--->PhoA
