Team:LCG-UNAM-Mexico:Journals:Uriel
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transform E. coli [https://2009.igem.org/Team:LCG-UNAM-Mexico/Resources/Strains#Expression_Strains BL21(DE3)plysS] from novagen. This strain uses a T7 phage polymerase that is controlled thorough Lac promoter. The multipromoter is composed of T7 & T3 promoters, we expect that our construction will respond to IPTG. | transform E. coli [https://2009.igem.org/Team:LCG-UNAM-Mexico/Resources/Strains#Expression_Strains BL21(DE3)plysS] from novagen. This strain uses a T7 phage polymerase that is controlled thorough Lac promoter. The multipromoter is composed of T7 & T3 promoters, we expect that our construction will respond to IPTG. | ||
- | Transformed cells were platted on LB medium with Kn and IPTG .5 mM | + | Transformed cells were platted on LB medium with Kn and IPTG .5 mM and expect that the colonies will fluoresce |
and could be seeing with a transluminator. | and could be seeing with a transluminator. | ||
Revision as of 02:53, 22 October 2009