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Team:Kyoto/CiC/Parts
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#[[Team:Kyoto/CiC|Cells in Cells]] | #[[Team:Kyoto/CiC|Cells in Cells]] | ||
#Parts | #Parts | ||
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</div><!-- /#topicpath --> | </div><!-- /#topicpath --> | ||
{{:Team:Kyoto/Leftnavi}} | {{:Team:Kyoto/Leftnavi}} | ||
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<div id="rightcontents"> | <div id="rightcontents"> | ||
| - | == | + | ==Parts== |
===Part design=== | ===Part design=== | ||
| - | In this project, we designed two | + | In this project, we designed two plasmids for RTS(Rapid Translation System, Roache); 1. HIV-TAT::(LALAAAA)3 expressing vector, 2.Signal for TIM23 complex::GFP expressing vector, and one plasmid for HeLa cells; Signal for TIM23 complex::EGFP expressing vector for HeLa cell. Both 1 and 3 have T7 promoter, terminator and their CDS between them. They can express each protein by RTS. Both parts are inserted in high copy number plasmid pSB1A2. 3. is made by inserting Signal for TIM23 complex into pEGFP-N3. |
===HIV-TAT-(LALAAAA)3-generator=== | ===HIV-TAT-(LALAAAA)3-generator=== | ||
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===TIM23complex signal::EGFP generator=== | ===TIM23complex signal::EGFP generator=== | ||
| - | [[Image: | + | [[Image:Figure(TIM23complex signal--EGFP generator).png|400px|center]] |
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| + | ===Signal for TIM23 complex::EGFP expressing vector for HeLa cells=== | ||
| + | |||
| + | [[Image:Figure(TIM23complex signal--EGFP generator)2.png|400px|center]] | ||
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| + | </div><!-- /#rightcontents --> | ||
Latest revision as of 02:58, 22 October 2009
Parts
Part design
In this project, we designed two plasmids for RTS(Rapid Translation System, Roache); 1. HIV-TAT::(LALAAAA)3 expressing vector, 2.Signal for TIM23 complex::GFP expressing vector, and one plasmid for HeLa cells; Signal for TIM23 complex::EGFP expressing vector for HeLa cell. Both 1 and 3 have T7 promoter, terminator and their CDS between them. They can express each protein by RTS. Both parts are inserted in high copy number plasmid pSB1A2. 3. is made by inserting Signal for TIM23 complex into pEGFP-N3.
HIV-TAT-(LALAAAA)3-generator
TIM23complex signal::EGFP generator
.png)
Signal for TIM23 complex::EGFP expressing vector for HeLa cells
2.png)
